Rosmarinic acid protects rats against post-stroke depression after transient focal cerebral ischemic injury through enhancing antioxidant response
Rosmarinic acid protects rats against post-stroke depression after transient focal cerebral ischemic injury through enhancing antioxidant response
复制标题
迷迭香酸通过增强抗氧化反应，保护大鼠短暂性局灶性脑缺血损伤后免受中风后抑郁的影响
DOI：
10.1016/j.brainres.2021.147336
复制
发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Fang Zongping
中科院分区：
文献类型：
--
作者：
Wang Jiajia;Wang Shiquan;Guo Haiyun;Li Yi;Jiang Zhenhua;Gu Ting;Su Binxiao;Hou Wugang;Zhong Haixing;Cheng D;an;Zhang Xijing;Fang Zongping
迷迭香酸（Rosmarinic acid，RA）是一种天然多酚类化合物，具有较强的抗氧化和抗炎活性。为探讨维甲酸对缺血性脑卒中及卒中后抑郁（PSD）的治疗作用，本实验采用大鼠脑缺血后给予不同剂量的维甲酸治疗。测量神经功能缺损和脑梗死体积。在不同时间点检测超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）的活性。此外，还进行强迫游泳试验和蔗糖偏好试验来检测RA的抗抑郁作用。我们的研究结果显示，RA管理显着减轻神经功能缺损和减少梗死体积。RA可减轻脑缺血半暗带SOD、CAT活性及GSH含量的下降。最重要的是，RA治疗减轻了抑郁行为。RA也可诱导Nrf 2表达增加，而Nrf 2-短发夹RNA序列下调Nrf 2可逆转RA诱导的SOD和CAT活性增加，以及对PSD的保护作用。本研究表明，RA对中风和PSD具有强大的神经保护作用，这可能是中风的一种有希望的治疗干预措施。
Rosmarinic acid (RA), a natural polyphenol, possesses potent antioxidant and anti-inflammatory activities. To evaluate the ability of RA to cure ischemic stroke and post-stroke depression (PSD), rats were treated with various doses of RA after cerebral ischemia. Neurological deficits and infarct volume of the brain were measured. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) were examined at different time points. In addition, a forced swimming test and sucrose preference test were performed to detect the anti-depressive effects of RA. Our results revealed RA administration significantly alleviated neurological deficits and reduced infarct volumes. RA attenuated the decrease of SOD, CAT activities and GSH levels in the ischemic penumbra of the brain. Most importantly, RA treatment alleviated the depression behaviors. Increased expression of Nrf2 was also induced by RA, while down regulation Nrf2 by Nrf2-short-hairpin RNA sequences reversed the increasing activity of SOD and CAT induced by RA, as well as the protection against PSD. The present study indicates that RA exerts a potent neuroprotective effect against stroke and PSD, which could be a promising therapeutic intervention for stroke.