Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
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DOI：
10.1083/jcb.200902117
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发表时间：
2009-10-19
影响因子：
7.8
通讯作者：
Rachubinski, Richard A.
中科院分区：
文献类型：
--
作者：
Chang, Jinlan;Mast, Fred D.;Rachubinski, Richard A.
在酿酒酵母中，过氧化物酶体从母细胞到芽的遗传是由V类肌球蛋白马达Myo2p进行的。然而，S.酿酒酵母Myo2p过氧化物酶体受体Inp2p在酿酒酵母科以外不易鉴定。在这里，我们证明了一个意想不到的作用Pex3蛋白在过氧化物酶体遗传。Pex3p和Pex3Bp都是过氧化物酶体整合膜蛋白，其通过与肌球蛋白球状尾直接相互作用而作为V类肌球蛋白的过氧化物酶体受体起作用。在缺乏Pex3Bp的细胞中，过氧化物酶体优先被母细胞保留，而大多数过氧化物酶体聚集并被转移到过表达Pex3Bp或Pex3p的细胞中的芽中。我们的研究结果揭示了一个前所未有的作用，Pex3蛋白家族的成员在过氧化物酶体运动和遗传除了他们的完善的作用，在内质网的过氧化物酶体生物合成。我们的研究结果指出过氧化物酶体的形成和遗传之间的时间联系，并描绘了在真核细胞中过氧化物酶体遗传的一般机制。
In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected role for Pex3 proteins in peroxisome inheritance. Both Pex3p and Pex3Bp are peroxisomal integral membrane proteins that function as peroxisomal receptors for class V myosin through direct interaction with the myosin globular tail. In cells lacking Pex3Bp, peroxisomes are preferentially retained by the mother cell, whereas most peroxisomes gather and are transferred en masse to the bud in cells overexpressing Pex3Bp or Pex3p. Our results reveal an unprecedented role for members of the Pex3 protein family in peroxisome motility and inheritance in addition to their well-established role in peroxisome biogenesis at the endoplasmic reticulum. Our results point to a temporal link between peroxisome formation and inheritance and delineate a general mechanism of peroxisome inheritance in eukaryotic cells.