An electrochemical stripping metalloimmunoassay based on silver-enhanced gold nanoparticle label
An electrochemical stripping metalloimmunoassay based on silver-enhanced gold nanoparticle label
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DOI：
10.1016/j.bios.2004.07.012
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发表时间：
2005-03-15
影响因子：
12.6
通讯作者：
Yu, RQ
中科院分区：
文献类型：
--
作者：
Chu, X;Fu, X;Yu, RQ
基于银在胶体金标记物上的沉淀，建立了一种新的、灵敏的电化学免疫分析方法。银金属在酸性溶液中溶解后，在玻碳电极上用阳极溶出伏安法(ASV)间接测定。以免疫球蛋白G(IgG)为模型，对该方法进行了非竞争性多相免疫分析。考察并优化了相关实验参数，包括抗原与抗体的反应时间、胶体金标记抗体的稀释度以及阳极溶出操作参数对峰电流的影响。阳极溶出峰电流与免疫球蛋白浓度在1.66~27.25µg·ml~(-1)范围内呈对数关系。人免疫球蛋白的检测下限为1ngml(-1)(即6×10(-12)M)，与比色酶联免疫吸附试验(ELISA)或基于Eu的荧光标记免疫分析方法相竞争。该方法的高性能归功于银(1)在玻碳电极上的灵敏ASV测定(检出限为5×10~(-9)M)，以及大量银在胶体金标记抗体上的催化沉淀。(C)2004爱思唯尔B.V.保留所有权利。
A novel, sensitive electrochemical immunoassay has been developed based on the precipitation of silver on colloidal gold labels which, after silver metal dissolution in an acidic solution, was indirectly determined by anodic stripping voltarmmetry (ASV) at a glassy-carbon electrode. The method was evaluated for a noncompetitive heterogeneous immunoassay of an immunoglobulin G (IgG) as a model. The influence of relevant experimental variables, including the reaction time of antigen with antibody, the dilution ratio of the colloidal gold-labeled antibody and the parameters of the anodic stripping operation, upon the peak current was examined and optimized. The anodic stripping peak current depended linearly on the IgG concentration over the range of 1.66 ng ml(-1) to 27.25 mug ml(-1) in a logarithmic plot. A detection limit as low as 1 ng ml(-1) (i.e., 6 x 10(-12) M) human IgG was achieved, which is competitive with colorimetric enzyme linked immuno-sorbent assay (ELISA) or with immunoassays based on fluorescent europium chelate labels. The high performance of the method is attributed to the sensitive ASV determination of silver (1) at a glassy-carbon electrode (detection limit of 5 x 10(-9) M) and to the catalytic precipitation of a large number of silver on the colloidal gold-labeled antibody. (C) 2004 Elsevier B.V. All rights reserved.