IGF2 antisense transcript expression in porcine postnatal muscle is affected by a quantitative trait nucleotide in intron 3
IGF2 antisense transcript expression in porcine postnatal muscle is affected by a quantitative trait nucleotide in intron 3
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DOI：
10.1016/j.ygeno.2004.09.006
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发表时间：
2004-12-01
期刊：
影响因子：
4.4
通讯作者：
Andersson, G
中科院分区：
文献类型：
--
作者：
Braunschweig, MH;Van Laere, AS;Andersson, G
父系表达的肌肉质量数量性状基因座 (QTL) 被映射到猪染色体 2p 远端的胰岛素样生长因子 II (IGF2) 基因座。最近证明，内含子 3-nt 3072 位置处的 G 到 A 转变是该 QTL 背后的数量性状核苷酸 (QTN)。在这项研究中，我们首次报道了印记猪 IGF2 反义转录本 (JGF2-AS) 的存在，并证明它们在出生后肌肉中的表达也受到 QTN 的影响。肌肉和肝脏中 IGF2 和 IGF2-AS RNA 的共同调节表达随着从胎儿到成年的转录减少而被证明。此外，具有不同QTL基因型的个体之间出生后肌肉中IGF2-AS表达的显着差异以及胎儿肌肉和肝脏中缺乏显着差异完全反映了IGF2有义转录本的发现。 (C) 2004 Elsevier Inc. 保留所有权利。
A paternally expressed quantitative trait locus (QTL) for muscle mass was mapped to the insulin-like growth factor II (IGF2) locus at the distal end of pig chromosome 2p. It was recently demonstrated that a G to A transition at position intron 3-nt 3072 is the quantitative trait nucleotide (QTN) underlying this QTL. In this study we report for the first time the existence of imprinted porcine IGF2 antisense transcripts (JGF2-AS) and demonstrate that their expression in postnatal muscle is also affected by the QTN. A coregulated expression of IGF2 and IGF2-AS RNAs in muscle and liver with decreasing transcription from fetal to adult age was demonstrated. Further, the significant difference found in expression of IGF2-AS in postnatal muscle between individuals with different QTL genotypes and the lack of significant differences in fetal muscle and liver reflect completely what has been found for IGF2 sense transcripts. (C) 2004 Elsevier Inc. All rights reserved.