Relationship between elevated soluble CD74 and severity of experimental and clinical ALI/ARDS.
Relationship between elevated soluble CD74 and severity of experimental and clinical ALI/ARDS.
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可溶性 CD74 升高与实验和临床 ALI/ARDS 严重程度之间的关系
DOI：
10.1038/srep30067
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发表时间：
2016-07-22
影响因子：
4.6
通讯作者：
Xia Z
中科院分区：
文献类型：
--
作者：
Wu G;Sun Y;Wang K;Chen Z;Wang X;Chang F;Li T;Feng P;Xia Z
CD74 在肺巨噬细胞的细胞表面表达，在急性肺损伤 (ALI) 中参与巨噬细胞迁移抑制因子 (MIF) 诱导的炎症反应。最近在自身免疫性肝病中发现了一种循环形式的 CD74（可溶性 CD74，sCD74）。使用两种小鼠ALI模型和细胞培养，我们检查了循环和肺泡腔中sCD74的存在，并初步评估了sCD74的生物学功能。小鼠 ALI 模型的血清和支气管肺泡灌洗液 (BALF) 中 sCD74 的浓度增加。 BALF 中 sCD74 水平升高与肺通透性和炎症呈正相关。此外，sCD74是巨噬细胞响应MIF刺激而分泌的，其本身可以刺激炎症细胞因子的产生。我们的临床研究证实了基础研究的一些发现。此外，我们还发现第 3 天血清 sCD74 水平与较差的临床结果相关。总之，较高的血清 sCD74 水平可能反映更严重的肺损伤，并可用于帮助医生确定急性呼吸窘迫综合征 (ARDS) 的预后。
CD74 is expressed on the cell surface of pulmonary macrophages and contributes to macrophage migration inhibitory factor (MIF)-induced inflammatory response in acute lung injury (ALI). A circulating form of CD74 (soluble CD74, sCD74) was recently discovered in autoimmune liver disease. Using two murine ALI models and cells culture, we examined the presence of sCD74 in circulation and alveolar space and preliminarily assessed the biological function of sCD74. The concentrations of sCD74 were increased in serum and bronchoalveolar lavage fluids (BALF) of murine ALI models. The elevated levels of sCD74 in BALF positively correlated with lung permeability and inflammation. In addition, sCD74 is secreted by macrophages in response to MIF stimulation and itself can stimulate the production of inflammatory cytokines. Our clinical study confirmed some findings of basic research. Moreover, we also found Day 3 serum sCD74 levels were associated with worse clinical outcomes. In conclusion, higher serum sCD74 levels may reflect more severe lung injury and may be used to help physicians determine prognosis of acute respiratory distress syndrome (ARDS).