COMPUTER-ANALYSIS OF THE BINDING REACTIONS LEADING TO A TRANSMEMBRANE RECEPTOR-LINKED MULTIPROTEIN COMPLEX INVOLVED IN BACTERIAL CHEMOTAXIS
COMPUTER-ANALYSIS OF THE BINDING REACTIONS LEADING TO A TRANSMEMBRANE RECEPTOR-LINKED MULTIPROTEIN COMPLEX INVOLVED IN BACTERIAL CHEMOTAXIS
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DOI：
10.1091/mbc.6.10.1367
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发表时间：
1995-10-01
影响因子：
3.3
通讯作者：
BOURRET, RB
中科院分区：
文献类型：
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作者：
BRAY, D;BOURRET, RB
细菌的趋化反应是由复合物介导的，复合物含有两个分子，每个分子是跨膜受体和细胞内信号蛋白CheA和CheW。突变体中的一个或其他的蛋白质的这种复合物是不存在的，无活性的，或在升高的量表达显示改变趋化行为和表型是难以解释的一些过表达突变体。我们已经研究了这些意想不到的表型可能来自导致活性复合物形成的结合步骤的可能性。一个有限的遗传算法被用来搜索的结合反应和相关的结合常数的集合，预期得到突变体表型在雅阁与实验数据。尝试了不同的结合平衡和关于特定受体复合物活性的不同假设。计算机分析表明，它是可能的，以获得与所观察到的表型相一致的结合平衡集，并提供了一个简单的解释，这些表型在各种条件下形成的活性和非活性复合物的分布。这种优化方法提供了一种基于行为数据分析活细胞内发生的反应的独特方法。
The chemotactic response of bacteria is mediated by complexes containing two molecules each of a transmembrane receptor and the intracellular signaling proteins CheA and CheW. Mutants in which one or the other of the proteins of this complex are absent, inactive, or expressed at elevated amounts show altered chemotactic behavior and the phenotypes are difficult to interpret for some overexpression mutants. We have examined the possibility that these unexpected phenotypes might arise from the binding steps that lead to active complex formation. A limited genetic algorithm was used to search for sets of binding reactions and associated binding constants expected to give mutant phenotypes in accord with experimental data. Different sets of binding equilibria and different assumptions about the activity of particular receptor complexes were tried. Computer analysis demonstrated that it is possible to obtain sets of binding equilibria consistent with the observed phenotypes and provided a simple explanation for these phenotypes in terms of the distribution of active and inactive complexes formed under various conditions. Optimization methods of this kind offer a unique way to analyze reactions taking place inside living cells based on behavioral data.