Role of mitotic control in spermatogenesis
Role of mitotic control in spermatogenesis
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DOI：
10.1016/s0015-0282(00)00603-8
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发表时间：
2000-08-01
影响因子：
6.7
通讯作者：
Lewis-Jones, DI
中科院分区：
文献类型：
--
作者：
Gazvani, MR;Wilson, EDA;Lewis-Jones, DI
目的：研究核型正常的不育男性和有生育能力的捐献者体细胞和精子中性染色体非整倍体的发生率。设计：一项前瞻性的二期对照研究。地点：生殖医学与医学遗传学教学医院。患者(S)：10例特发性少精子症患者和10例具有正常核型46，XY的捐精者。干预(S)：用含有α卫星DXZ1为X中心，DYZ1为Y长臂异染色区的多色荧光原位杂交(FISH)检测外周血淋巴细胞和精子主要观察指标(S)：两组患者外周血淋巴细胞和精子中X、Y和21号染色体非整倍体的发生率。结果：与X、Y和21号染色体相关的非整倍体在不育男性患者外周血淋巴细胞和精子中的发生率显著高于捐献者。所有男性体细胞染色体异倍体发生率与精子染色体异倍体发生率呈正相关。结论：这些发现为有丝分裂在精子发生中的重要性以及有丝分裂不稳定在不明原因少精子症中的作用提供了新的证据。(C)2000年，由美国生殖医学学会提供。
Objective: To study the correlation between the incidence of sex chromosome aneuploidies in the somatic cells and spermatozoa in karyotypically normal infertile men and fertile donors.Design: A prospective, phase two, controlled study.Setting: A teaching Hospital Reproductive Medicine and Medical Genetics Units.Patient(s): Ten patients with idiopathic oligozoospermia and 10 sperm donors with proven fertility, all with a normal karyotype 46,XY.Intervention(s): Multicolor fluorescence in situ hybridization (FISH) of peripheral blood lymphocytes and spermatozoa using a probe cocktail containing the alpha satellite DXZ1 for the X centromere, DYZ1 for the heterochromatic region of the long arm of the Y, and cosmids D21S259, D21S341, and D21S342 fur Down syndrome critical region of chromosome 21.Main Outcome Measure(s): The incidence of chromosome X, Y, and 21 aneuploidies in peripheral lymphocytes and spermatozoa in both groups.Result(s): The incidence of aneuploidies related to chromosomes X, Y, and 21 were significantly higher in peripheral lymphocytes and spermatozoa of infertile men compared with donors. There was a positive correlation between the incidence of chromosome aneuploidies in the somatic cells and sperm in all men.Conclusion(s): These findings provide suggestive evidence for the importance of mitosis in spermatogenesis and the role of mitotic instability in unexplained oligozoospermia. (C) 2000 by American Society for Reproductive Medicine.