Alternate primers for whole-genome SARS-CoV-2 sequencing.
Alternate primers for whole-genome SARS-CoV-2 sequencing.
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DOI：
10.1093/ve/veab006
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发表时间：
2021-01
期刊：
影响因子：
5.3
通讯作者：
V T Phan M
中科院分区：
文献类型：
--
作者：
Cotten M;Lule Bugembe D;Kaleebu P;V T Phan M
由于世界正在努力控制新型严重急性呼吸道综合征冠状病毒2（SARS-CoV-2），迫切需要制定有效的控制措施。病毒基因组序列中编码了重要信息，具有准确和完整的SARS-CoV-2序列，对于跟踪病毒的移动和演变以及指导开发疫苗和抗病毒药物的努力至关重要。虽然全球范围内进行了前所未有的SARS-CoV-2测序工作，但每月产生的基因组中约有19%至43%存在缺口，减少了其信息含量。目前的研究记录了基因组缺口频率及其在当前可用数据中的位置，并提供了一种替代引物组和测序方案，以帮助提高基因组的质量和覆盖率。
As the world is struggling to control the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), there is an urgency to develop effective control measures. Essential information is encoded in the virus genome sequence with accurate and complete SARS-CoV-2 sequences essential for tracking the movement and evolution of the virus and for guiding efforts to develop vaccines and antiviral drugs. While there is unprecedented SARS-CoV-2 sequencing efforts globally, approximately 19 to 43 per cent of the genomes generated monthly are gapped, reducing their information content. The current study documents the genome gap frequencies and their positions in the currently available data and provides an alternative primer set and a sequencing scheme to help improve the quality and coverage of the genomes.