The effect of 2-[(aminopropyl)amino] ethanethiol (WR-1065) on radiation induced DNA double strand damage and repair in V79 cells.
The effect of 2-[(aminopropyl)amino] ethanethiol (WR-1065) on radiation induced DNA double strand damage and repair in V79 cells.
复制标题
2-[(氨丙基)氨基]乙硫醇 (WR-1065) 对辐射诱导的 V79 细胞 DNA 双链损伤和修复的影响。
DOI：
10.1038/bjc.1987.97
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发表时间：
1987
影响因子：
8.8
通讯作者：
Grdina,DJ
中科院分区：
文献类型：
--
作者：
Sigdestad,CP;Treacy,SH;Knapp,LA;Grdina,DJ
辐射诱导的DNA双链断裂被认为是与细胞杀伤、诱导染色体畸变和致癌有关的重要病变。本文报道了放射性保护剂2-[（氨基丙基）氨基]乙硫醇（WR-1065）在pH 7.2或pH 9.6下对V79细胞辐射诱导的DNA损伤和修复的影响。WR-1065 （4mm）在钴-60伽马射线照射前和照射期间（用于剂量反应实验）或在修复时间测试期间（用于DNA重新连接实验）添加到培养基中。结果表明，WR-1065是一种有效的保护剂，可以防止辐射诱导的DNA双链断裂的形成。在pH为9.6和pH为7.2的实验中，WR-1065将DNA双链断裂因子分别降低了1.44和1.77。DNA双链重新连接的动力学取决于中性洗脱过程的pH值。与碱性洗脱得到的结果不同，在任何pH值下，WR-1065的存在都不影响DNA断裂的重新连接。
Radiation induced DNA double strand breaks are believed to be important lesions involved in processes related to cell killing, induction of chromosome aberrations and carcinogenesis. This paper reports the effects of the radioprotector 2-[(aminopropyl) amino] ethanethiol (WR-1065) on radiation-induced DNA damage and repair in V79 cells using the neutral elution method performed at pH 7.2 or pH 9.6. WR-1065 (4 mM) was added to the culture medium either 30 minutes prior to and during irradiation with Cobalt-60 gamma rays (for dose response experiments) or during the repair times tested (for DNA rejoining experiments). The results indicate that WR-1065 is an effective protector against the formation of radiation-induced double-strand breaks in DNA as measured using a neutral elution technique at either pH. The protector reduced the strand scission factors by 1.44 and 1.77 in experiments run at pH 9.6 and pH 7.2, respectively. The kinetics of DNA double-strand rejoining were dependent upon the pH at which the neutral elution procedure was performed. Unlike the results obtained with alkaline elution, rejoining of DNA breaks was unaffected by the presence of WR-1065 at either pH.