A role for melanin-concentrating hormone in the central regulation of feeding behaviour
A role for melanin-concentrating hormone in the central regulation of feeding behaviour
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DOI：
10.1038/380243a0
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发表时间：
1996-03-21
期刊：
影响因子：
64.8
通讯作者：
MaratosFlier, E
中科院分区：
文献类型：
--
作者：
Qu, DQ;Ludwig, DS;MaratosFlier, E
下丘脑在能量稳态和体重的综合调节中起着核心作用，许多下丘脑神经肽，如神经肽Y（参考文献1）、甘丙肽（参考文献2）、CRH（参考文献3）和GLP-1（参考文献4），都参与了这些作用的介导。为了发现参与体重调节的新的下丘脑肽，我们使用差异显示聚合酶链反应（5）来鉴定ob/+与ob/ob C57 Bl/6 J小鼠下丘脑中差异表达的信使RNA。我们在这里表明，一种mRNA在ob/ob小鼠下丘脑中过表达，编码神经肽黑色素浓集激素（MCH）。禁食进一步增加MCH mRNA在正常和肥胖动物中的表达。含有MCH的神经元位于下丘脑的前角和外侧。这些区域参与摄食行为的调节，但MCH在哺乳动物生理学中的作用尚不清楚。为了确定MCH是否参与摄食的调节，我们将MCH注射到大鼠的侧脑室中，发现它们的摄食量增加。这些结果表明，MCH参与下丘脑调节体重。
THE hypothalamus plays a central role in the integrated regulation of energy homeostasis and body weight, and a number of hypothalamic neuropeptides, such as neuropeptide Y (ref. 1), galanin(2), CRH (ref. 3), and GLP-1 (ref. 4), have been implicated in the mediation of these effects. To discover new hypothalamic peptides involved in the regulation of body weight, we used differential display polymerase chain reaction(5) to identify messenger RNAs that are differentially expressed in the hypothalamus of ob/+ compared with ob/ob C57Bl/6J mice. We show here that one mRNA that is overexpressed in the hypothalamus of ob/ob mice encodes the neuropeptide melanin-concentrating hormone (MCH). Fasting further increased expression of MCH mRNA in both normal and obese animals. Neurons containing MCH are located in the zona incerta and in the lateral hypothalamus. These areas are involved in regulation of ingestive behaviour, but the role of MCH in mammalian physiology is unknown. To determine whether MCH is involved in the regulation of feeding, we injected MCH into the lateral ventricles of rats and found that their food consumption increased. These findings suggest that MCH participates in the hypothalamic regulation of body weight.