Cytosolic Ca2+ under high glucose with suppressed Na+/K+ pump activity in rat sensory neurons
Cytosolic Ca2+ under high glucose with suppressed Na+/K+ pump activity in rat sensory neurons
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DOI：
10.1097/00001756-200401190-00038
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发表时间：
2004-01
期刊：
影响因子：
1.7
通讯作者：
M. Sanada;H. Matsuura;M. Omatsu-Kanbe;K. Sango;A. Kashiwagi;H. Yasuda
中科院分区：
文献类型：
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作者：
M. Sanada;H. Matsuura;M. Omatsu-Kanbe;K. Sango;A. Kashiwagi;H. Yasuda
采用荧光钙指示剂fura-2测定大鼠背根神经节（DRG）神经元胞浆内钙浓度（[Ca 2 +]i）。暴露于高（50 mM）细胞外K+引起[Ca 2 +]i的强烈增加，这几乎完全被尼索地平（10 μM）和芋螺毒素GVIA（10 μM）的同时存在所消除。尽管单独使用高D-葡萄糖（30 mM）或单独使用哇巴因（100 μM）并没有明显影响高K+诱导的[Ca 2 +]i升高，但用高D-葡萄糖和哇巴因预处理的神经元对高K+刺激表现出显着更大的[Ca 2 +]i反应，这几乎完全被尼索地平和芋螺毒素GVIA抑制。这些结果表明，高葡萄糖和抑制Na+/K+泵活性的组合增强了大鼠DRG神经元电压门控Ca 2+通道激活刺激的[Ca 2 +]i升高。
Cytosolic Ca2+ concentration ([Ca2+]i) was measured in isolated rat dorsal root ganglion (DRG) neurons using the fluorescent Ca2+ indicator fura-2. Exposure to high (50 mM) extracellular K+ evoked a robust increase in [Ca2+]i, which was almost totally abolished by concomitant presence of nisoldipine (10 μM) and -conotoxin GVIA (10 μM). Whereas either high (30 mM) D-glucose alone or ouabain (100 μM) alone did not appreciably affect the high K+-induced [Ca2+]i elevation, neurons pretreated with high D-glucose together with ouabain exhibited a significantly larger [Ca2+]i response to high K+ stimulation, which was almost completely inhibited by nisoldipine and -conotoxin GVIA. These results suggest that a combination of high glucose and suppressed Na+/K+ pump activity potentiates the [Ca2+]i elevation stimulated by activation of the voltage-gated Ca2+ channels in rat DRG neurons.