Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro
Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro
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DOI：
10.1007/s00705-015-2713-7
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发表时间：
2016-03-01
影响因子：
2.7
通讯作者：
Harding, Robert M.
中科院分区：
文献类型：
--
作者：
Higgins, Colleen M.;Bejerman, Nicolas;Harding, Robert M.
首次报道了所罗门群岛芋骨病相关病毒(CBDaV)的基因组序列。负链RNA基因组全长12,193个核苷酸，有6个主要开放阅读框架(ORF)，排列为3‘-N-P-P3-M-G-L-5’。在所有横纹病病毒中，典型的3‘前导序列和5’拖尾序列具有互补性。系统发育分析表明，CBDaV是杆状杆状病毒属的成员，这支持了先前关于病毒颗粒存在于骨骼感染的芋头细胞细胞质中的报道。CBDaV基因组序列的获得使评估该病毒在芋骨病中的作用成为可能，并确认该序列为柯罗卡西亚骨病病毒(CBDV)的序列。
We report the first genome sequence of a Colocasia bobone disease-associated virus (CBDaV) derived from bobone-affected taro [Colocasia esculenta L. Schott] from Solomon Islands. The negative-strand RNA genome is 12,193 nt long, with six major open reading frames (ORFs) with the arrangement 3'-N-P-P3-M-G-L-5'. Typical of all rhabdoviruses, the 3' leader and 5' trailer sequences show complementarity to each other. Phylogenetic analysis indicated that CBDaV is a member of the genus Cytorhabdovirus, supporting previous reports of virus particles within the cytoplasm of bobone-infected taro cells. The availability of the CBDaV genome sequence now makes it possible to assess the role of this virus in bobone, and possibly alomae disease of taro and confirm that this sequence is that of Colocasia bobone disease virus (CBDV).