Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution
Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution
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DOI：
10.1002/dvdy.20678
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发表时间：
2006-03-01
影响因子：
2.5
通讯作者：
Kamachi, Y
中科院分区：
文献类型：
--
作者：
Okuda, Y;Yoda, H;Kamachi, Y
B1组Sox基因编码在神经发育中起重要作用的HMG结构域转录因子。我们已经鉴定出6个斑马鱼B1 sox基因，包括泛脊椎动物sox1a/b、sox2和sox3，以及鱼类特有的sox19a/b。SOX19A/B蛋白表现出与其他B1 SOX蛋白相似的转录激活潜能。sox19a和sox3的表达开始于胚胎发生的大约1000个细胞阶段，并在屏蔽期局限于未来的外胚层。这与羊膜中Sox2和/或Sox3的外胚层表达相似。随着发育的进展，这6个B1 - sox基因显示出独特的表达模式，从一个区域到另一个区域明显重叠。sox19a在早期神经外胚层中广泛表达，类似于羊膜动物的泛神经Sox2表达，而斑马鱼的Sox2则表现为前限制性表达。比较基因组学表明，sox19a/b与哺乳动物Sox15 （G组）具有同源关系，B1/G Sox基因是通过两轮基因组复制从一个共同的祖先基因产生的。因此，似乎每个B1/G Sox基因在脊椎动物进化过程中获得了不同的表达谱和功能。
Group B1 Sox genes encode HMG domain transcription factors that play major roles in neural development. We have identified six zebrafish B1 sox genes, which include pan-vertebrate sox1a/b, sox2, and sox3, and also fish-specific sox19a/b. SOX19A/B proteins show a transcriptional activation potential that is similar to other B1 SOX proteins. The expression of sox19a and sox3 begins at approximately the 1,000-cell stage during embryogenesis and becomes confined to the future ectoderm by the shield stage. This is reminiscent of the epiblastic expression of Sox2 and/or Sox3 in amniotes. As development progresses, these six B1 sox genes display unique expression patterns that overlap distinctly from one region to another. sox19a expression is widespread in the early neuroectoderm, resembling pan-neural Sox2 expression in amniotes, whereas zebrafish sox2 shows anterior-restricted expression. Comparative genomics suggests that sox19a/b and mammalian Sox15 (group G) have an orthologous relationship and that the B1/G Sox genes arose from a common ancestral gene through two rounds of genome duplication. It seems likely, therefore, that each B1/G Sox gene has gained a distinct expression profile and function during vertebrate evolution.