Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.
Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.
复制标题
DOI：
10.1021/acs.nanolett.5b03331
复制
发表时间：
2016-01-13
期刊：
影响因子：
10.8
通讯作者：
Wanunu M
中科院分区：
文献类型：
--
作者：
Henley RY;Ashcroft BA;Farrell I;Cooperman BS;Lindsay SM;Wanunu M
人们假设，核糖体通过探测tRNA物种之间的结构差异，在蛋白质翻译过程中获得额外的保真度。我们通过直径约3 nm的合成纳米孔测量了不同tRNA物种的转运动力学。每种tRNA物种在从平衡状态变形的时间尺度上各不相同，如在蛋白质翻译的转位步骤中。利用机器学习算法，我们可以区分五种tRNA物种，分析tRNA二元混合物的比例，并区分tRNA同分受体。
It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ~3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors.