An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts.
An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: I. Normal fibroblasts.
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小鼠牙周膜成纤维细胞胶原分泌的电子显微镜放射自显影研究：I.正常成纤维细胞。
DOI：
10.1002/ar.1092010402
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Garant,PR
中科院分区：
文献类型：
--
作者：
Cho,MI;Garant,PR
电子显微镜放射自显影分析显示，粗面内质网（RER）的3 H-脯氨酸标记在3分钟时达到最大值，高尔基囊1和2在10分钟时达到最大值。20分钟的高尔基体囊泡为3型，30分钟的分泌前颗粒和分泌颗粒。细胞外胶原基质的标记发生在30分钟时，并随时间增加。这些观察结果表明，牙周膜成纤维细胞中胶原蛋白的前α链在RER中合成，并在10分钟内运输到高尔基体。然后这些链在高尔基囊2型中进行平行排列，并在10至20分钟之间在高尔基囊3型中形成节段长间距样微晶。分泌前颗粒和成熟分泌颗粒在30分钟时少量标记的峰值和在30分钟时开始的细胞外胶原基质标记的快速增加，表明分泌颗粒的形成需要大约30分钟，并且在牙周膜成纤维细胞中存在分泌颗粒移位的快速系统。这一证据进一步支持了先前发表的牙周膜成纤维细胞中胶原分泌的微管依赖性系统的形态学证据（Cho和Garant，1981 b）。
Analysis of electon microscopic radioautographs revealed a maximum labeling with3H‐proline of rough endoplasmic reticulum (RER) at 3 minutes, Golgi saccules 1 and 2 at 10 minutes. Golgi saccules type 3 at 20 minutes, and presecretory and secretory granules at 30 minutes. Labeling of the extra‐cellular collagen matrix occurred at 30 minutes and increased with time. These observations suggest that pro‐a‐chains of collagen in periodontal ligament fibroblasts are synthesized in the RER and transported to the Golgi apparatus within 10 minutes. These chains then undergo parallel alignment in Golgi saccules type 2 and form segment‐long‐spacing‐like crystallites in Golgi saccules type 3 between 10 and 20 minutes. The peak labeling of presecretory granules and mature secretory granules in small amounts at 30 minutes and the rapid increase in labeling of extracellular collagen matrix which begins at 30 minutes, indicates that the formation of secretory granules requires approximately 30 minutes and that a rapid system of secretory granule translocation exists in periodontal ligament fibroblasts. This evidence futher supports the previously published morphologic evidence for a microtubuledependent system of collagen secretion in periodontal ligament fibroblasts (Cho and Garant, 1981b).