Expression of secretory phospholipase A2s in human atherosclerosis development
Expression of secretory phospholipase A2s in human atherosclerosis development
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DOI：
10.1016/j.atherosclerosis.2006.08.062
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发表时间：
2008-01-01
期刊：
影响因子：
5.3
通讯作者：
Ishii, Toshiharu
中科院分区：
文献类型：
--
作者：
Kimura-Matsumoto, Masayo;Ishikawa, Yukio;Ishii, Toshiharu
分泌型磷脂酶A2（sPLA 2）有助于磷脂的水解。其中，sPLA 2-IIA、sPLA 2-V和sPLA 2-X被认为是动脉内膜中脂质积聚的增强剂。然而，其他类型的sPLA 2在人主动脉壁中的分布和产生尚不清楚。因此，在本研究中，七种类型的sPLA 2，包括IIA，IID，IIE，IIF，III，V和X在人主动脉粥样硬化的发展中的分布和生产进行了全面的免疫组织化学和原位杂交（ISH）。sPLA 2-IIF在正常主动脉中未见表达，但sPLA 2-IIF表达随动脉粥样硬化的发展而增加。免疫双染显示sPLA 2-V仅在平滑肌细胞表达，而其它sPLA 2在巨噬细胞和平滑肌细胞中均有表达。使用sPLA 2 cDNA的ISH显示，每个mRNA的表达模式与每个相应sPLA 2的免疫组织化学结果一致。这些结果表明，七种sPLA 2在动脉粥样硬化发展的各个阶段以不同的模式表达，并可能通过降解磷脂发挥致动脉粥样硬化作用。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Secretory phospholipase A2s (sPLA2s) contribute to the hydrolysis of phospholipid. Among them, sPLA2-IIA, -V, and -X have been regarded as enhancers of lipid accumulation in arterial intima. However, the distribution and production of the other types of sPLA2 in human aortic wall remain unclear. Therefore, in this study, the distribution and production of seven types of sPLA2 including IIA, IID, IIE, IIF, III, V, and X in atherosclerosis development in the human aorta were comprehensively examined by immunohistochemistry and in situ hybridization (ISH). The extent of sPLA2s expression increased with atherosclerosis development, but only sPLA2-IIF was never observed in the normal aorta. Double-immunostaining demonstrated that sPLA2-V expression was limited to smooth muscle cells (SMCs), although the other sPLA2s were expressed in both macrophages and SMCs. ISH using sPLA2 cDNAs revealed that the expression pattern of each mRNA was consistent with the results of immunohistochemistry for each corresponding sPLA2. These results indicate that the seven types of sPLA2 are expressed with various patterns in all stages of atherosclerosis development and may play an atherogenic role through degradation of phospholipid. (C) 2007 Elsevier Ireland Ltd. All rights reserved.