Alu and L1 sequence distributions in Xq24-q28 and their comparative utility in YAC contig assembly and verification.
Alu and L1 sequence distributions in Xq24-q28 and their comparative utility in YAC contig assembly and verification.
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Xq24-q28 中的 Alu 和 L1 序列分布及其在 YAC 重叠群组装和验证中的比较实用性。
DOI：
10.1006/geno.1993.1205
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Schlessinger,D
中科院分区：
文献类型：
--
作者：
Porta,G;Zucchi,I;Hillier,L;Green,P;Nowotny,V;D'Urso,M;Schlessinger,D
通过Southern印迹分析已经通过其他方法组装并定位在Xq 24-q28内的YAC重叠群来评估含有Alu和L1的TaqI限制性片段的含量。重叠群中YACs的指纹图谱一致，利用基于M. V. Olsonet al. (1986，Proc. Natl. Acad. Sci. USA 83：7826）来分析关于片段大小的数字化数据，指纹识别本身可以在435个YAC的测试组中的约40%之间建立匹配。在100 kb的分辨率，这两个重复的元素被发现在整个区域，没有明显的富集oflavin或L1的DNA中的G相比，发现在R带。然而，与随机总体分布一致，高达100 kb的划界区域包含重复元件簇。局部浓度可能有助于解释已报道的ORF 1和L1探针与中期染色体片段的差异杂交。
The contents ofAlu- and L1-containingTaqI restriction fragments were assessed by Southern blot analyses across YAC contigs already assembled by other means and localized within Xq24-q28. Fingerprinting patterns of YACs in contigs were concordant, and using software based on that of M. V. Olsonet al.(1986,Proc. Natl. Acad. Sci. USA83: 7826) to analyze digitized data on fragment sizes, fingerprinting itself could establish matches among about 40% of a test group of 435 YACs. At 100-kb resolution, both repetitive elements were found throughout the region, with no apparent enrichment ofAluor L1 in DNA of G compared to that found in R bands. However, consistent with a random overall distribution, delimited regions of up to 100 kb contained clusters of repetitive elements. The local concentrations may help to account for the reported differential hybridization ofAluand L1 probes to segments of metaphase chromosomes.