A protein nuclear extract from D. melanogaster larval tissues
A protein nuclear extract from D. melanogaster larval tissues
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DOI：
10.4161/fly.5392
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发表时间：
2007-11-01
期刊：
影响因子：
1.2
通讯作者：
Corona, Davide F. V.
中科院分区：
文献类型：
--
作者：
La Rocca, Gaspare;Burgio, Giosalba;Corona, Davide F. V.
蛋白质核抽提物的制备通常是研究真核细胞细胞核中发生的体外生物过程的第一步。核抽提物已被广泛应用于不同的模式生物中，以鉴定和研究细胞核中的蛋白质功能。果蝇胚胎可以大量收集，并已成为生产蛋白质核提取物的首选来源。然而，果蝇体内蛋白质功能的研究大多是在幼虫组织中进行的。在这里，我们报告了一种新的方法，从整个果蝇幼虫中提取高度稳定的大规模蛋白质核提取物，适用于各种生化分析。
Preparation of protein nuclear extracts is often the first step to study in vitro biological processes occurring in the nucleus of the eukaryotic cell. Nuclear extracts have been extensively used in different model organisms to identify and study protein function in nuclei. Drosophila embryos can be collected in large quantities and have been the source of choice for the production of protein nuclear extracts. However, most of Drosophila in vivo studies on protein function are conducted in larval tissues. Here we report a new method to produce highly stable large-scale protein nuclear extracts from whole Drosophila larvae that are suited for a variety of biochemical analyses.