Investigation of Protein Detection Parameters Using Nanofunctionalized Organic Field-Effect Transistors
Investigation of Protein Detection Parameters Using Nanofunctionalized Organic Field-Effect Transistors
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DOI：
10.1021/nn305903q
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发表时间：
2013-05-01
期刊：
影响因子：
17.1
通讯作者：
Bao, Zhenan
中科院分区：
文献类型：
--
作者：
Hammock, Mallory L.;Knopfmacher, Oren;Bao, Zhenan
使用有机场效应晶体管（OFFERT）的生物检测在食品安全、环境监测和医疗诊断等多种应用中获得越来越多的关注。然而，仍然缺乏一个全面的，经验性的研究OFET传感器的基本限制。在本文中，我们提出了一个深入的研究各种参数影响的生物传感使用的OFET装饰与金纳米粒子（金纳米粒子）的结合位点。这些参数包括受体之间的间距、缓冲液的pH和缓冲液的离子强度。为此，我们采用凝血酶蛋白及其相应的DNA结合适体，形成我们的模型检测系统。我们证明了这种蛋白质的检测限为100 pM，对其他蛋白酶具有高选择性。我们在这里描述了一种可行的方法，蛋白质检测与OFFERT和一个彻底的调查参数管理的生物检测事件使用OFFERT。我们得到的结果应该提供重要的指导方针，以定制传感器的动态范围，以适应其他所需的OFET为基础的生物检测应用。
Biodetection using organic field-effect transistors (OFETs) is gaining increasing interest for applications as diverse as food security, environmental monitoring, and medical diagnostics. However, there still lacks a comprehensive, empirical study on the fundamental limits of OFET sensors. In this paper, we present a thorough study of the various parameters affecting biosensing using an OFET decorated with gold nanoparticle (AuNP) binding sites. These parameters include the spacing between receptors, pH of the buffer, and ionic strength of the buffer. To this end, we employed the thrombin protein and its corresponding DNA binding aptamer to form our model detection system. We demonstrate a detection limit of 100 pM for this protein with high selectivity over other proteases in situ. We describe herein a feasible approach for protein detection with OFETs and a thorough investigation of parameters governing biodetection events using OFETs. Our obtained results should provide important guidelines to tailor the sensors dynamic range to suit other desired OFET-based biodetection applications.