Effects of the Combined Stress Induced by Stocking Density and Feed Restriction on Hematological and Cytokine Parameters as Stress Indicators in Laying Hens
Effects of the Combined Stress Induced by Stocking Density and Feed Restriction on Hematological and Cytokine Parameters as Stress Indicators in Laying Hens
复制标题
DOI：
10.5713/ajas.2011.10315
复制
发表时间：
2011-03-01
期刊：
影响因子：
--
通讯作者：
Jang, In-Surk
中科院分区：
文献类型：
--
作者：
Kang, Sun-Young;Ko, Young-Hyun;Jang, In-Surk
研究了高饲养密度和限饲复合应激源对蛋鸡白细胞分类计数和细胞因子表达等免疫学参数的影响。将40只白色来航产蛋鸡随机分为对照组(12 kg体重/m~2)和应激组(44 kg体重/m~(2))，应激组蛋鸡每天按对照组自愿采食量的75%饲喂，连续12天。在联合应激源作用12天后，体重显著下降，但不影响肝和脾的相对重量。血液学指标中异嗜性白细胞(H)、淋巴细胞(L)、单核细胞、H/L比值两组间差异无统计学意义。在细胞因子方面，应激组大鼠肝脏脂多糖诱导的肿瘤坏死因子-α(LITNF-α)和诱导型一氧化氮合酶(INOS)的表达水平显著高于对照组(p<；0.05)。然而，白介素4和白介素6在肝脏中的表达水平不受联合应激源的影响。与对照组相比，联合应激组大鼠脾组织中LITNF-α的表达显著上调(p<；0.05)。而复合应激源对脾组织IL-4、IL-6和iNOS的表达无明显影响。综上所述，高饲养密度和限饲复合应激源增加了鸟类淋巴和非淋巴器官中的一些促炎细胞因子，包括LITNF-α和iNOS，提示对特定应激源的细胞因子表达的改变可以作为评估鸟类应激反应的另一个参数。
A study was conducted to investigate the effects of the combined stressor induced by high stocking density with feed restriction on immunological parameters such as leukocyte differential counts and cytokine expression in laying hens. A total of forty White Leghorn laying hens were randomly allotted into the control (12 kg of body weight/m(2)) and the stress (44 kg of body weight/m(2)) groups, and then birds of the stress group were given 75% of voluntary intake of the control birds for 12-d on a daily basis. There was a significant decrease in body weight without affecting the relative weights of the liver and spleen after 12-d of the combined stressor. In hematological values, no significant difference in leukocyte differential counts including heterophils (H), lymphocytes (L), monocytes and H:L ratio was observed between the two groups. In cytokines, hepatic lipopolysaccharide-induced tumor necrosis factor-alpha (LITNF-alpha) and inducible nitric oxide synthase (iNOS) expression levels in the stress group were significantly (p < 0.05) higher compared with those in the control group. However, the expression levels of interleukin (IL)-4 and IL-6 in the liver were not affected by the combined stressor. Splenic LITNF-alpha expression in the combined stressor group was significantly (p < 0.05) up-regulated compared with that in the control birds. However, the combined stressor did not affect splenic IL-4, IL-6 and iNOS expression. In conclusion, the combined stressor caused by high stocking density with feed restriction enhanced some pro-inflammatory cytokines including LITNF-alpha and iNOS in lymphoid and non-lymphoid organs of birds, suggesting that altered cytokine expression to given stressors can be another parameter that can be used in assessing stress responses of birds.