Instantaneous isotropic volumetric imaging of fast biological processes
Instantaneous isotropic volumetric imaging of fast biological processes
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DOI：
10.1038/s41592-019-0393-z
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发表时间：
2019-06-01
期刊：
影响因子：
48
通讯作者：
Prevedel, Robert
中科院分区：
文献类型：
--
作者：
Wagner, Nils;Norlin, Nils;Prevedel, Robert
在细胞分辨率下捕捉高度动态的生物过程是生物学中反复出现的挑战。在这里，我们展示了将选择体积照明与同时采集正交光场相结合，产生具有高各向同性空间分辨率的三维图像，并显著减少了重建伪影，从而克服了目前光场显微镜实现的局限性。我们展示了青竹心脏和血流成像的单细胞分辨率和无运动伪影，体积速率高达200赫兹。
To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.