The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord.
The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord.
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DOI：
10.11480/jmds.550109
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发表时间：
2008-03
影响因子：
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通讯作者：
K. Fukushima;M. Enomoto;S. Tomizawa;Makoto Takahashi;Y. Wakabayashi;S. Itoh;Y. Kuboki;K. Shinomiya
中科院分区：
文献类型：
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作者：
K. Fukushima;M. Enomoto;S. Tomizawa;Makoto Takahashi;Y. Wakabayashi;S. Itoh;Y. Kuboki;K. Shinomiya
我们研制了一种不同孔径的蜂窝状冻干型I型人工晶状体胶原(Honeycomb Collagen：HC)，并检测了蜂窝状胶原对神经再生的影响。我们分析了背根神经节(DRG)外植体在HC上的突起生长，包括在体外和将HC直接植入成年大鼠脊髓缺损处的体内。HC上背根神经节的神经突起通过毛孔呈线状延伸。400微孔的HC促进了神经突起的延伸，YIGSR层粘连蛋白多肽涂层的HC比纤维连接蛋白涂层延长了更多的神经突起。将YIGSR涂层的HC支架植入T8~9节段脊髓2 mm缺损处。植入四周后，植入物已经降解，并被自身组织取代，修复了受伤的部位。在植入区观察到神经丝阳性纤维，穿过HC和脊髓残端之间的边界。在功能上，植入10周后，在股四头肌观察到运动诱发电位。电生理检查显示植入物上的轴突束重建。这一结果表明，我们开发的蜂窝形状比随机毛孔海绵形状更有利于宿主脊髓，并促进脊髓损伤后的轴突再生。
We developed a honeycomb-shaped lyophilized Type I atelocollagen (Honeycomb Collagen: HC) with different pore sizes, and the effectiveness of the honeycomb shape on nerve regeneration was examined. We analyzed neurite outgrowth of dorsal root ganglion (DRG) explants on HC, both in vitro and, with direct implantation of HC into the defects of adult rat spinal cords, in vivo. The neurites of DRGs on HC extended linearly through the pores. HC with a 400 microm-pore size enhanced neurite extension, and YIGSR laminin peptide coating to the HC extended more neurites than fibronectin coating. The HC scaffolds coated with YIGSR were implanted into 2 mm-defects of spinal cords at the level of T8-9. Four weeks after implantation, the implants had degraded and been replaced with self-tissues, repairing the injured site. Neurofilament-positive fibers were observed in the implantation area and passed the borders between the HC and spinal cord stumps. Functionally, a motor-evoked potential was observed in the quadriceps femoris muscle 10 weeks after implantation. The electrophysiological examination showed reconstruction of axon tracts over the implant. This result indicates that our developed honeycomb shape is advantageous for host spinal cord compared to the random pored sponge shape, and that it promotes axonal regeneration after spinal cord injury.