Sara endosomes and the asymmetric division of intestinal stem cells
Sara endosomes and the asymmetric division of intestinal stem cells
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DOI：
10.1242/dev.104240
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发表时间：
2014-05-01
期刊：
影响因子：
4.6
通讯作者：
Gonzalez-Gaitan, Marcos
中科院分区：
文献类型：
--
作者：
Montagne, Chrystelle;Gonzalez-Gaitan, Marcos
组织稳态由成体干细胞维持，成体干细胞自我更新并产生分化细胞。具有不同命运的子细胞的产生是由来自外部小生境的信号分子介导的，或者在干细胞有丝分裂时在两个子细胞之间不对称地分派。在成年果蝇中肠，肠干细胞（ISC）分裂产生新的ISC和肠母细胞（EB）分化的子代。限制于EB的Notch信号传导活性调节肠细胞命运决定。在这里，我们表明，ISCs分裂不对称，和萨拉在ISCs内体是专门派遣到推定的EB。在ISC有丝分裂过程中，Notch和Delta通过Sara内体进行运输，从而导致Notch信号偏差，如Sara突变体中所揭示的：Sara本身有助于控制ISC不对称分裂。我们的数据揭示了ISC有丝分裂过程中的内在内体机制，该机制参与了成体肠谱系的维持。
Tissue homeostasis is maintained by adult stem cells, which self-renew and give rise to differentiating cells. The generation of daughter cells with different fates is mediated by signalling molecules coming from an external niche or being asymmetrically dispatched between the two daughters upon stem cell mitosis. In the adult Drosophila midgut, the intestinal stem cell (ISC) divides to generate a new ISC and an enteroblast (EB) differentiating daughter. Notch signalling activity restricted to the EB regulates intestinal cell fate decision. Here, we show that ISCs divide asymmetrically, and Sara endosomes in ISCs are specifically dispatched to the presumptive EB. During ISC mitosis, Notch and Delta traffic through Sara endosomes, thereby contributing to Notch signalling bias, as revealed in Sara mutants: Sara itself contributes to the control of the ISC asymmetric division. Our data uncover an intrinsic endosomal mechanism during ISC mitosis, which participates in the maintenance of the adult intestinal lineage.