Identification of a novel SEREX antigen, SLC2A1/GLUT1, in esophageal squamous cell carcinoma.
Identification of a novel SEREX antigen, SLC2A1/GLUT1, in esophageal squamous cell carcinoma.
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DOI：
10.3892/ijo.28.2.463
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发表时间：
2006-02
影响因子：
5.2
通讯作者：
Mari Kuboshima;H. Shimada;Tianxiang Liu;K. Nakashima;F. Nomura;M. Takiguchi;T. Hiwasa;T. Ochiai
中科院分区：
文献类型：
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作者：
Mari Kuboshima;H. Shimada;Tianxiang Liu;K. Nakashima;F. Nomura;M. Takiguchi;T. Hiwasa;T. Ochiai
我们进行了SEREX（serological identification of antigens by recombinant cDNA expression cloning），并确定SLC 2A 1（solute carrier family 2/assisted glucose transporter，member 1）为食管鳞状细胞癌（SCC）患者血清IgG抗体识别的抗原。血清抗SLC 2A 1抗体（s-SLC 2A 1-Abs）的水平，采用细菌表达的谷胱甘肽-S-转移酶-SLC 2A 1融合蛋白酶联免疫吸附试验检测，食管鳞癌患者显著高于健康供体。当使用截止水平作为健康供体的平均值± 2x标准差时，57名SCC患者中共有12名（21%）显示为s-SLC 2A 1-Ab阳性。s-SLC 2A 1-Abs的存在与临床病理因素或生存率无关。由于s-SLC 2A 1-Abs与其他常规血清标志物的阳性无关，因此s-SLC 2A 1-Abs与这些常规血清标志物的联合检测可能有助于食管SCC的诊断和监测。
We have carried out SEREX (serological identification of antigens by recombinant cDNA expression cloning), and identified SLC2A1 (solute carrier family 2/facilitated glucose transporter, member 1) as an antigen recognized by serum IgG antibodies in patients with esophageal squamous cell carcinoma (SCC). The levels of serum anti-SLC2A1 antibodies (s-SLC2A1-Abs), examined by enzyme-linked immunosorbent assay using bacterially expressed glutathione-S-transferase-SLC2A1 fusion protein, were significantly higher in patients with esophageal SCC than in healthy donors. When using a cut-off level as the mean + 2x standard deviations of healthy donors, a total of 12 (21%) out of 57 SCC patients were revealed as positive for s-SLC2A1-Abs. The presence of s-SLC2A1-Abs was not associated with either clinicopathological factors or survival. Because s-SLC2A1-Abs were not associated with the positivity of other conventional serum markers, a combination assay of s-SLC2A1-Abs with these conventional serum markers may be useful for the diagnosis and monitoring of esophageal SCC.