Arabidopsis cell culture for comparable physiological and genetic studies
Arabidopsis cell culture for comparable physiological and genetic studies
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拟南芥细胞培养用于可比较的生理和遗传学研究
DOI：
10.1080/15592324.2020.1781384
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发表时间：
2020
影响因子：
2.9
通讯作者：
Desaki Yoshitake
中科院分区：
文献类型：
--
作者：
Maruyama Shingo;Shibuya Naoto;Kaku Hanae;Desaki Yoshitake
从不同植物物种建立的细胞培养已被用于一系列的生理和生化研究。与整株培养相比，细胞培养中细胞类型和簇大小的均质性往往能给出更清晰和更简单的结果。另一方面，细胞系之间的生理条件和对外界刺激的反应可能存在差异，这对比较研究可能是有问题的。为了将植物细胞培养的实用性与拟南芥丰富的信息和遗传资源相结合，我们系统地研究了建立可用于各种遗传资源比较研究的细胞系的方法/条件。从成熟种子分生组织中建立的拟南芥细胞系表现出可重复性和可比性的MamP反应，如ROS的产生和防御相关基因的表达。培养细胞的MAMP反应表现出特异性，取决于相应的MAMP受体的存在或不存在。用蛋白激酶抑制剂K252a进行的药理学研究也表明，细胞培养对这类研究是有用的。这些结果表明，该方法用于建立拟南芥细胞系是有用的，这对遗传资源之间的比较研究是有用的。
Cell cultures established from various plant species have been used for a range of physiological and biochemical studies. Homogeneity of cell types and size of clusters in the cell culture often gave a clearer and simpler results compared to those obtained with the whole plant. On the other hand, possible variability of physiological conditions and responsiveness to external stimuli between the cell lines could be problematic for comparative studies. Aiming at combining the usefulness of plant cell culture with the rich information and genetic resources of Arabidopsis, we systemically examined the methods/conditions to establish cell lines for comparative studies, which could be applicable to a variety of genetic resources. Arabidopsis cell lines thus established from the meristem of mature seeds showed reproducible and comparable MAMP responses such as ROS generation and defense-related gene expression. MAMP responses of the cultured cells showed the specificity depending on the presence/absence of the corresponding MAMP receptor. Pharmacological study with a protein kinase inhibitor, K252a, also showed the usefulness of the cell culture for such studies. These results indicated the usefulness of the method to establish Arabidopsis cell lines, which are useful for comparative studies between genetic resources.
开发丁香假单胞菌-拟南芥悬浮细胞感染系统，用于研究 III 型分泌和模式触发免疫的宿主代谢依赖性调节。
DOI：
10.1094/mpmi-10-18-0295-fi
发表时间：
2019
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Qing Yan;Conner J. Rogan;Jeffrey C. Anderson
通讯作者：
Jeffrey C. Anderson
影响因子：
4.9
作者：
Desaki, Yoshitake;Miya, Ayako;Shibuya, Naoto
通讯作者：
Shibuya, Naoto
影响因子：
4.9
作者：
Desaki, Yoshitake;Takahashi, Shohei;Shibuya, Naoto
通讯作者：
Shibuya, Naoto