Evaluation of Different Assay Systems for Identification of Environmental Aeromonas Strains
Evaluation of Different Assay Systems for Identification of Environmental Aeromonas Strains
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环境气单胞菌菌株鉴定的不同检测系统的评价
DOI：
10.1128/aem.51.6.1373-1373.1986
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发表时间：
1986
影响因子：
4.4
通讯作者：
J. Barja
中科院分区：
文献类型：
--
作者：
A. E. Toranzo;Ysabel Santos;Teresa P. Nieto;J. Barja
将常规测试中的重要生化反应与两个多项测试系统API-20E(AnalytabProducts，Plainview，NY)的对应反应进行了比较。和嗜水气单胞菌，评价其对运动性气单胞菌鉴定的准确性。与鱼隔绝的。共检出气单胞菌49株。Voges-Proskauer试验、吲哚产生、明胶酶活力、GAS产生、阿拉伯糖发酵、赖氨酸脱羧酶反应均呈假阴性或阳性反应。在三个鉴定系统中，甘露醇和肌醇的发酵以及精氨酸二水解酶和鸟氨酸脱羧酶试验都有很好的相关性。嗜水气单胞菌培养基检出气体失败表明，该培养基不完全适合于确定产气或厌氧菌株。根据本研究的结果，我们认为环境气单胞菌的鉴定方法和分类方案是可行的。必须是标准化的。
Important biochemical reactions in conventional tests were compared with counterpart reactions in two multiple test systems, API-20E (Analytab Products, Plainview, N.Y.) and Aeromonas hydrophila medium, to evaluate their accuracy for the identification of motile Aeromonas spp. isolated from fish. In a total of 49 Aeromonas spp. isolates and 10 A. hydrophila reference strains, false-negative or -positive reactions were detected in the Voges-Proskauer test, indole production, gelatinase activity, production of gas, fermentation of arabinose, and lysine decarboxylase reaction. A good correlation was found, among the three identification systems, for the fermentation of mannitol and inositol as well as for the arginine dihydrolase and ornithine decarboxylase tests. The failure of A. hydrophila medium in the detection of gas indicates that this medium is not entirely suitable for defining aerogenic or anaerogenic strains. From the results of the present study, we consider that of the identification method and taxonomic scheme to be adopted for environmental Aeromonas spp. must be standardized.