Function of bacterial community dynamics in the formation of cadaveric semiochemicals during in situ carcass decomposition
Function of bacterial community dynamics in the formation of cadaveric semiochemicals during in situ carcass decomposition
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DOI：
10.1111/1462-2920.13828
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发表时间：
2017-08
影响因子：
5.1
通讯作者：
J. Pascual;Christian von Hoermann;Ann-Marie Rottler-Hoermann;Omer Nevo;Alicia M Geppert;J. Sikorski;Katharina J. Huber;Sandra Steiger;M. Ayasse;J. Overmann
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文献类型：
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作者：
J. Pascual;Christian von Hoermann;Ann-Marie Rottler-Hoermann;Omer Nevo;Alicia M Geppert;J. Sikorski;Katharina J. Huber;Sandra Steiger;M. Ayasse;J. Overmann
死亡的哺乳动物组织的分解涉及一个复杂的时间顺序的epinecrotic细菌。微生物的活动可能会释放出不同的尸体挥发性有机化合物，这些化合物反过来又会吸引尸体分解的其他关键参与者，如食腐昆虫。为了阐明动物尸体上的表皮坏死细菌的动态和潜在功能，我们通过结合高通量Illumina 16 S rRNA测序和挥发物的气相色谱-质谱分析，监测了在不同森林生态系统中孵育的死产仔猪上的细菌群落。我们的研究结果表明，群落结构的epinecrotic细菌和类型的尸体挥发性化合物的分解过程中释放的确定性，而不是随机过程。个别尸体的挥发性有机化合物与特定的类群在第一阶段的分解，这是由细菌为主。通过最佳拟合的多元线性回归模型，乙酸，吲哚和苯酚的合成可以联系到肠杆菌科，Tissierellaceae和黄单胞菌科的活性，分别。这些结论也是相称的代谢所描述的优势类群确定这些家庭。尸体挥发性有机化合物的可预测性原位合成的epinecrotic细菌为未来的化学生态学和法医学研究提供了新的基础。
The decomposition of dead mammalian tissue involves a complex temporal succession of epinecrotic bacteria. Microbial activity may release different cadaveric volatile organic compounds which in turn attract other key players of carcass decomposition such as scavenger insects. To elucidate the dynamics and potential functions of epinecrotic bacteria on carcasses, we monitored bacterial communities developing on still-born piglets incubated in different forest ecosystems by combining high-throughput Illumina 16S rRNA sequencing with gas chromatography-mass spectrometry of volatiles. Our results show that the community structure of epinecrotic bacteria and the types of cadaveric volatile compounds released over the time course of decomposition are driven by deterministic rather than stochastic processes. Individual cadaveric volatile organic compounds were correlated with specific taxa during the first stages of decomposition which are dominated by bacteria. Through best-fitting multiple linear regression models, the synthesis of acetic acid, indole and phenol could be linked to the activity of Enterobacteriaceae, Tissierellaceae and Xanthomonadaceae, respectively. These conclusions are also commensurate with the metabolism described for the dominant taxa identified for these families. The predictable nature of in situ synthesis of cadaveric volatile organic compounds by epinecrotic bacteria provides a new basis for future chemical ecology and forensic studies.