A transcription factor FgSte12 is required for pathogenicity in Fusarium graminearum
A transcription factor FgSte12 is required for pathogenicity in Fusarium graminearum
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DOI：
10.1111/mpp.12155
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Gu, Qin;Zhang, Chengqi;Ma, Zhonghua
与酵母Fus3/Kss1交配/丝化途径同源的保守的丝裂原活化蛋白激酶(MAPK)参与了禾谷镰刀菌营养发育和致病力的调节。然而，人们对该途径的下游转录因子知之甚少。在酿酒酵母中，同源结构域蛋白STE12是由Fus3/Kss1激活的关键转录因子。在本研究中，我们鉴定了禾谷镰刀菌中的STE12同源基因FgSte12。FgSTE12缺失突变体(FgSte12)虽然在菌丝生长、分生孢子形成和脱氧雪腐镰刀菌烯醇(DON)生物合成方面没有表现出明显的表型变化，但在毒力和纤维素酶和蛋白酶的分泌方面受到了损害。此外，FgSte12和FgGPMK1(FUS3/KSS1相关的MAPK基因)突变体有几个共同的表型特征。此外，我们还发现FgGpmk1控制着FgSte12的核定位。酵母双杂交和亲和捕获实验表明，FgSte12与FgSte11-Ste7-Gpmk1复合体相互作用。综上所述，这些结果表明FgSte12是FgSte11-Ste7-Gpmk1的下游靶标，在禾谷镰刀菌的致病过程中起着重要作用。
A conserved mitogen-activated protein kinase (MAPK) cascade homologous to the yeast Fus3/Kss1 mating/filamentation pathway is involved in the regulation of vegetative development and pathogenicity in Fusarium graminearum. However, little is known about the downstream transcription factors of this pathway. In Saccharomyces cerevisiae, the homeodomain protein Ste12 is a key transcription factor activated by Fus3/Kss1. In this study, we characterized a Ste12 orthologue FgSte12 in F.graminearum. The FgSTE12 deletion mutant (FgSte12) was impaired in virulence and in the secretion of cellulase and protease, although it did not show recognizable phenotype changes in hyphal growth, conidiation or deoxynivalenol (DON) biosynthesis. In addition, FgSte12 and the FgGPMK1 (a FUS3/KSS1-related MAPK gene) mutant shared several phenotypic traits. Furthermore, we found that FgGpmk1 controls the nuclear localization of FgSte12. Yeast two-hybrid and affinity capture assays indicated that FgSte12 interacts with the FgSte11-Ste7-Gpmk1 complex. Taken together, these results indicate that FgSte12 is a downstream target of FgSte11-Ste7-Gpmk1 and plays an important role in pathogenicity in F.graminearum.