Efficient and sequence-specific DNA-templated polymerization of peptide nucleic acid aldehydes
Efficient and sequence-specific DNA-templated polymerization of peptide nucleic acid aldehydes
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DOI：
10.1021/ja038058b
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发表时间：
2003-11-19
影响因子：
15
通讯作者：
Liu, DR
中科院分区：
文献类型：
--
作者：
Rosenbaum, DM;Liu, DR
基于DNA模板还原胺化反应的距离依赖性和D。林恩和同事，我们开发了合成肽核酸（PNA）醛的DNA模板聚合。偶联反应以高效和序列特异性的方式进行，即使在不同序列的PNA醛的混合物存在下。有效地形成含有多达40个PNA单元（代表10个连续偶联反应）的合成肽核酸聚合物。制备含有定制官能团的PNA的容易性以及这些发现提高了通过翻译、选择、PCR扩增和多样化的迭代循环来进化合成序列定义的聚合物的可能性，所述迭代循环以前仅适用于生物大分子。
On the basis of the distance-dependence of DNA-templated reductive amination reactions and of recent findings of D. Lynn and co-workers, we developed DNA-templated polymerizations of synthetic peptide nucleic acid (PNA) aldehydes. The coupling reactions proceed in a highly efficient and sequence-specific manner, even in the presence of mixtures of PNA aldehydes of different sequence. Synthetic peptide nucleic acid polymers containing as many as 40 PNA units (representing 10 consecutive coupling reactions) were formed efficiently. The ease of preparing PNAs containing tailor-made functional groups together with these findings raises the possibility of evolving synthetic sequence-defined polymers by iterated cycles of translation, selection, PCR amplification, and diversification previously available only to biological macromolecules.