Impact of adipose-derived stem cells on engineering hair follicle germ-like tissue grafts for hair regenerative medicine
Impact of adipose-derived stem cells on engineering hair follicle germ-like tissue grafts for hair regenerative medicine
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DOI：
10.1016/j.jbiosc.2021.02.001
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发表时间：
2021-06-04
影响因子：
2.8
通讯作者：
Fukuda, Junji
中科院分区：
文献类型：
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作者：
Nakajima, Rikuma;Tate, Yoshiki;Fukuda, Junji
毛发再生医学已成为一种有前途的治疗策略，严重脱发，如终末期雄激素性脱发。已经探索了各种工程三维组织移植物的方法，因为它们在移植时驱动毛囊再生的能力。在本研究中，我们证明了人脂肪源性干细胞（hASCs）组装成毛囊胚芽（HFG）样聚集体，用于从头毛发再生。我们将人毛乳头细胞（hDPC）、鼠胚胎上皮细胞和hASC在悬浮液中混合，并使它们形成聚集体。在三天的培养过程中，细胞最初形成具有三种细胞类型的随机分布的单个聚集体，但上皮细胞和毛乳头细胞随后彼此分离并形成哑铃形HFG，hASC位于hDPC聚集体侧。与没有hASC的HFG相比，hASC的参与显著增加了与毛发形态发生相关的基因表达。在我们的可扩展的实验室制作的芯片设备中观察到三种细胞类型的自组织。含有hASC的HFG在移植到裸鼠后有效地产生毛干，而用不含hASC的HFG仅产生少数毛干。这种方法可能是一个有前途的策略，制造组织移植的头发再生医学。(C)2021年，生物技术学会，日本。All rights reserved.
Hair regenerative medicine has emerged as a promising treatment strategy for severe hair loss, such as end-stage androgenetic alopecia. Various approaches to engineering three-dimensional tissue grafts have been explored since they drive the ability to regenerate hair follicles when transplanted. In the present study, we demonstrated the assembly of human adipose-derived stem cells (hASCs) into hair follicle germ (HFG)-like aggregates for de novo hair regeneration. We mixed human dermal papilla cells (hDPCs), murine embryonic epithelial cells, and hASCs in suspension, and allowed them to form aggregates. During three days of culture, cells initially formed a single aggregate with a random distribution of the three cell types, but the epithelial and dermal papilla cells subsequently separated from each other and formed a dumbbell-shaped HFG, with hASCs localized on the hDPC aggregate side. The involvement of hASCs significantly increased gene expression associated with hair morphogenesis compared to HFGs without hASCs. The self-organization of the three cell types was observed in our scalable lab-made chip device. HFGs containing hASCs efficiently generated hair shafts upon transplantation to nude mice, while only a few shafts were generated with HFGs without hASCs. This approach may be a promising strategy for fabricating tissue grafts for hair regenerative medicine. (C) 2021, The Society for Biotechnology, Japan. All rights reserved.