HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH MERCURY CATHODE ELECTROCHEMICAL DETECTION - APPLICATION TO LIPID HYDROPEROXIDE ANALYSIS
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH MERCURY CATHODE ELECTROCHEMICAL DETECTION - APPLICATION TO LIPID HYDROPEROXIDE ANALYSIS
复制标题
DOI：
10.1016/0378-4347(95)00182-4
复制
发表时间：
1995-08-18
影响因子：
3
通讯作者：
GIROTTI, AW
中科院分区：
文献类型：
--
作者：
KORYTOWSKI, W;GEIGER, PG;GIROTTI, AW
使用反相高效液相色谱法和汞滴阴极还原模式电化学检测器[HPLC-ED（Hg）]，可以以高灵敏度和特异性分析脂质过氧化氢物质。本研究的目的是检查HPLC-ED（Hg）操作中的不同变量，并选择最佳条件分析几种生物相关的过氧化物，包括来自胆固醇、胆固醇亚油酸酯、油酸酯、亚油酸酯和两种合成磷脂酰胆碱的物质。参数，如操作电位和流动相溶剂的比例，电解质的组成，和离子强度进行了评价，为每个过氧化物类。在最佳条件下，我们实现了四种胆固醇氢过氧化物物质的基线分离，不仅彼此分离，而且与磷脂氢过氧化物分离;胆固醇和磷脂氢过氧化物的检测限分别为<0.3pmol和< 30 pmol。
Lipid hydroperoxide species can be analyzed with high sensitivity and specificity, using reversed-phase highperformance liquid chromatography with reductive mode electrochemical detection on a mercury drop cathode [HPLC-ED(Hg)]. The purpose of this study was to examine different variables in the operation of HPLC-ED(Hg) and to select optimal conditions for the analysis of several biologically relevant peroxides, including species derived from cholesterol, cholesteryl linoleate, oleate, linoleate, and two synthetic phosphatidylcholines. Parameters such as operating potential and mobile-phase solvent proportions, electrolyte composition, and ionic strength were evaluated for each peroxide class. tinder optimal conditions, we have achieved baseline separation of four cholesterol hydroperoxide species, not only from one another, but also from phospholipid hydroperoxides; detection limits were < 0.3 pmol and < 30 pmol for the cholesterol and phospholipid hydroperoxides, respectively.