Single Cell Isolation on a Centrifugal Flow Disk with Integrated Tandem Microchambers
Single Cell Isolation on a Centrifugal Flow Disk with Integrated Tandem Microchambers
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DOI：
10.1166/sl.2008.540
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发表时间：
2008-12-01
期刊：
影响因子：
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通讯作者：
Kubo, Izumi
中科院分区：
文献类型：
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作者：
Furutani, Shunsuke;Nagai, Hidenori;Kubo, Izumi
单细胞分析单细胞中的各种事件需要定量测量相关因子来描述细胞功能。然而，使用传统技术进行这样的测量太困难和耗时。我们提出了一种先进的方法，同时分离大量的细胞使用“串联微室”的离心流动盘。为了实现单细胞分析的高通量，我们的目标是在细胞分析中普遍使用高度集成的微室来分离和包埋单细胞反应室。对于不同大小的细胞，例如微生物或Jurkat细胞，用相同的盘和条件进行40-200个颗粒/μ L的单细胞分离。因此，这些结果证明了我们的离心流盘分离具有不同大小的各种细胞的可能性。单细胞分离的效率依赖于泊松分布，本研究中分离的细胞数量与理论基本一致。
Single cell analysis of various events in single cell requires quantitative measurement of associated factors to describe cellular function. However, such measurements are too difficult and time-consuming to perform using traditional techniques. We propose an advanced method for simultaneous isolation of a large number of cells using "tandem micro chambers" on a centrifugal flow disk. In order to realize the high throughput of single cell assay, we aim the common use of highly integrated micro chambers for the isolation and entrapment of a single cell reaction chambers in cell assay. With cell different in size such as microorganisms or Jurkat cell, single cell isolation for 40-200 particles/mu L was performed with the same disk and condition. Thus, the possibility that various kinds of cells which have different sizes would be isolated by our centrifugal flow disk was demonstrated by these results. The efficiency of single cell isolation was depended on Poisson distribution and the number of the isolated cells in this study was almost consistent with the theory.