Controlling performance of lipase immobilised on bioinspired silica
Controlling performance of lipase immobilised on bioinspired silica
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DOI：
10.1039/c2tb00462c
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Patwardhan, Siddharth V.
中科院分区：
文献类型：
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作者：
Forsyth, Claire;Patwardhan, Siddharth V.
脂肪酶(南极洲假丝酵母脂肪酶B)被固定在二氧化硅上，通过一种温和的路线使用最近开发的一种生物启发的二氧化硅形成方法来生产。与传统的固定化方法相比，该固定化方法具有操作简单、条件温和、成本低、一步法操作简单、制备时间短等优点。脂肪酶被选为酶，因为它在工业上的广泛应用，特别是在食品和制药行业。与其他使用生物灵感二氧化硅包埋酶的方法相比，我们证明了所生产的生物催化剂的性能有几个显著的改进。在优化的条件下获得了非常高的固定化效率(接近100%)。固定化的酶也表现出高水平的活性，超过了之前报道的脂肪酶在生物启发的二氧化硅上固定化的研究。此外，我们还展示了通过调节二氧化硅载体的比表面积、平均孔径和平均粒径来实现对酶活性的控制。固定化酶还具有很好的重复使用潜力，并表现出更好的热稳定性和pH稳定性。生物催化剂在湍流条件下表现良好，表现出良好的存储性能，并成功地用于台式反应器，因此有力地支持了它们在商业应用中的应用。固定化酶的性能也与工业基准Novozym(R)435相当或更好。使用生物启发的固定化方法比目前的方法有显著的好处，这些因素可能会增加工业上的潜在应用。
Lipase (Candida antarctica lipase B) was immobilised on silica that was produced via a mild route using a recently developed biologically inspired silica formation method. This route of immobilisation was favourable compared to traditional methods due to its simplicity, mild conditions, low cost, one-step procedure and short preparation time. Lipase was chosen as the enzyme due to its wide implementation in industry, particularly in the food and pharmaceutical industries. Compared to other methods using bioinspired silica entrapment of enzymes, we demonstrate several significant improvements in the performance of the biocatalysts produced. Very high immobilisation efficiencies (close to 100%) were achieved under optimised conditions. The immobilised enzymes also displayed high levels of activity, which exceeded those reported in previous studies of lipase that was immobilised on bioinspired silica. Furthermore, we demonstrate the control over enzyme activity which was achieved through the entrapment conditions by regulating surface area, the average pore diameter and the mean particle size of the silica support. The immobilised enzymes also had very good reuse potential, and showed improved thermal and pH stability. The biocatalysts also performed well in turbulent conditions, showed good storage properties and were successfully used in bench-top reactors, therefore strongly supporting their use in commercial applications. The immobilised enzymes also performed comparably to or better than the industrial benchmark, Novozym (R) 435. The bioinspired method of immobilisation used has significant benefits over current methods, and these factors could increase potential applications in industry.