Micropropagation of 'wild pear' Pyrus pyrifolia (Burm F.) Nakai. I. Explant establishment and shoot multiplication.
Micropropagation of 'wild pear' Pyrus pyrifolia (Burm F.) Nakai. I. Explant establishment and shoot multiplication.
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DOI：
10.15835/nbha361108
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发表时间：
2008
影响因子：
1.8
通讯作者：
A. Thakur;J. Kanwar
中科院分区：
文献类型：
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作者：
A. Thakur;J. Kanwar
该研究旨在标准化野梨的体外外植体建立和芽增殖技术。春季观察到最高的外植体建立频率（77.88%），与冬季的外植体建立频率（76.21%）相当。雨季期间观察到外植体建立最低（61.94%）。茎尖的建立频率（69.07%）显着低于节段的建立频率（71.95%）。抗褐变剂减少了外植体的褐变，但无法消除它。抗褐变剂的作用程度取决于外植体收集的季节和外植体的类型。与褐变发生率相对较高的雨季、夏季和秋季相比，防褐变剂在褐变发生率较低的春季和冬季更有用。虽然，每升 2.0 mg BA 时获得了最高数量的芽（11.24 个/培养物），但该 BA 水平并未产生所需长度（> 2.0 cm）的芽。 BA（1.5 毫克/升）+ IBA（0.5 毫克/升）是野梨芽增殖的最佳生长调节剂组合，因为它产生足够数量的芽（每个培养物 10.21 个芽）和理想的芽长度。使用木本植物培养基 (WPM) 获得的每个培养物的芽数量最多 (11.20)，其次是 MS 培养基 (10.21)。使用 MS 培养基观察到最高的芽长（3.21 厘米），与 WPM 相当。
The study was undertaken to standardize in vitro explant establishment and shoot multiplication technique for wild pear. The highest explant establishment (77.88%) was observed during spring, which was on a par with explant establishment frequency during winter season (76.21%). The lowest explant establishment was observed during rainy season (61.94%). The establishment frequency for shoot tips (69.07%) was significantly less than the establishment frequency for nodal segments (71.95%). The antibrowning agents decreased explant browning, yet they could not eliminate it. The magnitude of the effect of the antibrowning agents depended on the season of explant collection and type of explant. Antibrowning agents were more useful during spring and winter seasons when the incidence of browning was low, in comparison to rainy, summer and autumn seasons when the browning was comparatively high. Though, the highest number of shoots (11.24/ culture) were obtained with 2.0 mg per litre BA, but this BA level did not produce shoots of desirable length (>2.0 cm). BA (1.5 mg per litre) + IBA (0.5 mg per litre) was the best growth regulator combination for shoot multiplication in wild pear as it produced sufficient number of shoots (10.21 shoots per culture) having desirable shoot length. The highest number of shoots per culture (11.20) was obtained with Woody Plant Medium (WPM), followed by MS medium (10.21). The highest shoot length (3.21 cm) was observed with MS Medium and it was on a par with WPM.