Elastic chitosan conduits with multiple channels and well defined microstructure.
Elastic chitosan conduits with multiple channels and well defined microstructure.
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DOI：
10.1016/j.ijbiomac.2012.04.022
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发表时间：
2012-07
影响因子：
8.2
通讯作者：
Jixiang Zhu;Y. Xiong;Chen-guang Zeng;Na Qiang;Daping Quan;J. Wan
中科院分区：
文献类型：
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作者：
Jixiang Zhu;Y. Xiong;Chen-guang Zeng;Na Qiang;Daping Quan;J. Wan
采用冷冻干燥法制备了四种具有纵向多通道和内部微结构可控的壳聚糖导管。其中一个导管由壳聚糖溶液（ab NC）制成，而其他三个组分别由使用京尼平作为化学交联剂（ab gNC）、磷酸氢二钠作为物理交联剂（ab pNC）或组合的离子和共价共交联剂（ab gpNC）的预胶化壳聚糖溶液制成。壳聚糖导管的孔隙率范围为88%至90%。与NC、gNC和pNC相比，gpNC显示出高度互连和均匀分布的孔。相比之下，gNC和gpNC在37°C PBS溶液中显示约10%的体积溶胀比，尽管gpNC支架的吸水率最高，超过其原始质量的17倍。压缩测试表明，gpNC具有显著的弹性，即使在将它们压缩到原始高度的20%后，也能保持其物理完整性。gpNC的弹性模量达到80kPa，是其他组的2倍多。通过MTT和SEM观察，PC12细胞在壳聚糖gpNC支架上的粘附和增殖表现出良好的特性，表明gpNC支架具有应用于神经组织工程的潜力。
Four kinds of chitosan conduits with longitudinal multi-channels and controlled internal microstructures were prepared using a special mold and a freeze-drying method. One of the conduits was fabricated from a chitosan solution (ab NC), while the other three groups were made from a pre-gelled chitosan solution using genipin as a chemical cross-linker (ab gNC), dibasic sodium phosphate as a physical cross-linker (ab pNC) or a combined ionic and covalent co-cross-linker (ab gpNC), respectively. The porosity of the chitosan conduits ranged from 88 to 90%. The gpNC showed highly interconnected and uniformly distributed pores compared to NC, the gNC and pNC. In contrast, the gNC and gpNC showed about 10% of the volume swelling ratio in 37°C PBS solution, although the gpNC scaffold's water uptake was the highest, at more than 17 times its original mass. Compressive tests showed that gpNC had significant elasticity and maintained its physical integrity even after compressing them down to 20% of their original height. The elastic modulus of gpNC reached 80kPa, which was more than twice that of the other groups. Adhesion and proliferation of PC12 cells on chitosan gpNC scaffolds showed excellent properties by MTT and SEM observation, which indicated the potential of gpNC scaffolds for nerve tissue engineering applications.