Electroacupuncture improves restraint stress-induced delay of gastric emptying via central glutaminergic pathways in conscious rats
Electroacupuncture improves restraint stress-induced delay of gastric emptying via central glutaminergic pathways in conscious rats
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DOI：
10.1016/j.neulet.2005.11.069
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发表时间：
2006-05-15
影响因子：
2.5
通讯作者：
Takahashi, Toku
中科院分区：
文献类型：
--
作者：
Iwa, Masahiro;Nakade, Yukiomi;Takahashi, Toku
针灸已用于治疗功能性胃肠道（GI）疾病。动物研究表明，针灸可以改善各种压力引起的生理反应。我们研究了电针 (EA) ST-36（足三里；下肢）对应激引起的胃排空延迟的影响。与对照组（64 +/- 2.1%，n = 8）相比，束缚应激显着延迟了 90 分钟内的固体食物胃排空（27.3 +/- 2.1%，n = 8）。通过脑池内 (IC) 注射 GABA(A) 受体拮抗剂、荷包牡丹碱甲碘 (46.5 +/- 3.1%; n = 6) 和 GABA(B) 受体拮抗剂苯氯芬 (phaclofen) (48 +/- 3.3%; n = 6)，抑制应激引起的胃排空延迟得到显着恢复。 ST-36 时的 EA 显着改善了约束应激引起的胃排空延迟 (49.7 +/- 1.4%)。通过 IC 注射谷氨酸受体拮抗剂犬尿酸 (30.1 +/- 2.1%) 进行预处理，可以防止 EA 对应激引起的胃排空延迟的刺激作用。总之，束缚应激引起的胃排空延迟是通过中枢 GABAA 和 GABAB 受体介导的。 ST-36 处的 EA 刺激脑干中的谷氨酰胺能神经元，从而改善压力引起的胃排空延迟。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Acupuncture has been used for treating functional gastrointestinal (GI) disorders. Animal studies demonstrated that acupuncture improves various stress-induced physiological responses. We investigated the effects of electroacupuncture (EA) at ST-36 (Zusanli; lower limb) on stress-induced delay of gastric emptying. Solid food gastric emptying in 90 min was significantly delayed by restraint stress (27.3 +/- 2.1%, n = 8), compared to that of controls (64 +/- 2.1%, n = 8). Restraint stress-induced delay of gastric emptying was significantly restored by the intracisternal (IC)-injection of GABA(A) receptor antagonist, bicuculline methiodide (46.5 +/- 3.1%; n = 6) and GABA(B) receptor antagonist, phaclofen (48 +/- 3.3%; n = 6). Delayed gastric emptying induced by restraint stress was significantly improved by EA at ST-36 (49.7 +/- 1.4%). The stimulatory effect of EA on stress-induced delay of gastric emptying was prevented by pretreatment with IC-injection of glutamate receptor antagonist, kynurenic acid (30.1 +/- 2.1%). In conclusion, restraint stress-induced delay of gastric emptying is mediated via central GABAA and GABAB receptors. EA at ST-36 stimulates glutaminergic neurons in the brainstem resulting in improvement of stress-induced delay of gastric emptying. (c) 2005 Elsevier Ireland Ltd. All rights reserved.