Analysis of elevated temperature-induced inhibition of photosystem II using chlorophyll a fluorescence induction kinetics in wheat leaves (Triticum aestivum)
Analysis of elevated temperature-induced inhibition of photosystem II using chlorophyll a fluorescence induction kinetics in wheat leaves (Triticum aestivum)
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DOI：
10.1111/j.1438-8677.2009.00319.x
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发表时间：
2011-01-01
期刊：
影响因子：
3.9
通讯作者：
Bharti, S.
中科院分区：
文献类型：
--
作者：
Mathur, S.;Jajoo, A.;Bharti, S.
小麦是世界上许多地区的主要农作物。研究了高温诱导的小麦光合效率的变化。aestivum）叶片叶绿素a荧光诱导动力学。离体叶片经受35 ℃、40 ℃或45 ℃的高温胁迫。参数，如Fv/Fm，性能指数（PI），和反应中心吸光度比（RC/ABS）推导出使用径向图从荧光诱导曲线与植物效率分析仪（PEA）。为了获得关于荧光诱导动力学的精确信息，使用biolyzer hp 3软件绘制能量管道叶模型。在35摄氏度，没有影响光合效率，包括放氧复合物，PSII的供体侧仍然活跃。在40 ℃时，活性降低了14%，而在45 ℃时，观察到K中间步骤，表明对放氧复合物的不可逆损伤。该分析结果可用于田间高温胁迫下小麦活力和抗逆性的快速筛选。
Wheat is the major crop plant in many parts of the world. Elevated temperature-induced changes in photosynthetic efficiency were studied in wheat (T. aestivum) leaves by measuring Chl a fluorescence induction kinetics. Detached leaves were subjected to elevated temperature stress of 35 degrees C, 40 degrees C or 45 degrees C. Parameters such as Fv/Fm, performance index (PI), and reaction centre to absorbance ratio (RC/ABS) were deduced using radial plots from fluorescence induction curves obtained with a plant efficiency analyser (PEA). To derive precise information on fluorescence induction kinetics, energy pipeline leaf models were plotted using biolyzer hp3 software. At 35 degrees C, there was no effect on photosynthetic efficiency, including the oxygen-evolving complex, and the donor side of PSII remained active. At 40 degrees C, activity was reduced by 14%, while at 45 degrees C, a K intermediate step was observed, indicating irreversible damage to the oxygen-evolving complex. This analysis can be used to rapidly screen for vitality and stress tolerance characteristics of wheat growing in the field under high temperature stress.