An interleukin 4-induced DNase I hypersensitive site indicates opening of the gamma 1 switch region prior to switch recombination.
An interleukin 4-induced DNase I hypersensitive site indicates opening of the gamma 1 switch region prior to switch recombination.
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白细胞介素 4 诱导的 DNase I 超敏感位点表明伽马 1 开关区域在开关重组之前打开。
DOI：
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发表时间：
1989
影响因子：
4.4
通讯作者：
Andreas Radbruch
中科院分区：
文献类型：
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作者：
J. Schmitz;Andreas Radbruch
白细胞介素4（IL 4）指导类转换重组到有丝分裂原激活的鼠B淋巴细胞中的γ 1转换区（s γ 1）。未重排的s γ 1及其5'侧翼区的转录在开关重组之前由IL 4诱导的发现已经证明了IL 4通过选择性地诱导γ 1开关区对通用开关重组酶的可接近性来促进s mu/s γ 1开关重组的想法。在此，通过显示在不到6小时内IL 4诱导活化的鼠B细胞中与s γ 1区5'相邻的DNA酶I超敏（HS）位点，提供了该模型的进一步证据。染色质结构的这种变化很可能反映了HS位点内或附近的调节蛋白的IL 4响应性结合。该结果提供了一个候选序列的IL 4依赖性增强子/启动子的转录γ 1。
Interleukin 4 (IL4) directs class switch recombination to the gamma 1 switch region (s gamma 1) in mitogen-activated murine B lymphocytes. The finding that transcription of the unrearranged s gamma 1 and its 5' flanking region is induced by IL4 prior to switch recombination had argued for the idea that IL4 promotes s mu/s gamma 1 switch recombination by selectively inducing accessibility of the gamma 1 switch region for a general switch recombinase. Here further evidence for this model is provided by showing that within less than 6 h IL4 induces a DNase I hypersensitive (HS) site 5' adjacent to the s gamma 1 region in activated murine B cells. This change in chromatin structure most likely reflects the IL4-responsive binding of regulatory proteins within or in the vicinity of the HS site. The result provides a candidate sequence for an IL4-dependent enhancer/promoter of transcription of s gamma 1.