Small RNA Sequencing: A Technique for miRNA Profiling
Small RNA Sequencing: A Technique for miRNA Profiling
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DOI：
10.1007/978-1-4939-7614-0_21
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Ozanne, Susan E.
中科院分区：
文献类型：
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作者：
Pantaleao, Lucas Carminatti;Ozanne, Susan E.
鉴定动物组织中的microRNA（miRNA）特征是评估健康或疾病中基因表达的转录后调控研究的重要第一步。小RNA测序（sRNA-Seq）是下一代基于测序的技术，目前被认为是最强大和通用的miRNA分析工具。在这里，我们描述了sRNA-Seq协议，包括从哺乳动物组织中纯化RNA，文库制备和原始数据分析。
Identifying microRNA (miRNA) signatures in animal tissues is an essential first step in studies assessing post-transcriptional regulation of gene expression in health or disease. Small RNA sequencing (sRNA-Seq) is a next-generation sequencing-based technology that is currently considered the most powerful and versatile tool for miRNA profiling. Here, we describe a sRNA-Seq protocol including RNA purification from mammalian tissues, library preparation, and raw data analysis.