Up-converting phosphor technology-based lateral flow assay for detection of Schistosoma circulating anodic antigen in serum
Up-converting phosphor technology-based lateral flow assay for detection of Schistosoma circulating anodic antigen in serum
复制标题
DOI：
10.1128/jcm.00877-07
复制
发表时间：
2008-01-01
影响因子：
9.4
通讯作者：
van Dam, Govert J.
中科院分区：
文献类型：
--
作者：
Corstjens, Paul L. A. M.;van Lieshout, Lisette;van Dam, Govert J.
血吸虫环状阳极抗原（CAA）血清浓度反映患者的实际虫负荷，是人群筛查和流行病学研究的重要工具。然而，对于个体输入性血吸虫病病例的诊断，目前的酶联免疫吸附试验（ELISA）缺乏敏感性和稳健性。因此，我们建立了一种检测血清CAA的横向流动（LF）方法，用于输入性活动性血吸虫感染的个体诊断。与标准ELISA方法相比，应用荧光亚微米级上转换荧光粉技术（UPT）报告粒子提高了分析灵敏度。UPT-LF测试与选择40个特征流行病学样本的评估表明，信号强度和感染强度之间存在良好的相关性。随后，将UPT-LF测定法应用于166例荷兰居民（移民和旅行者）疑似血吸虫病的血清样本，在这种情况下，群体常规抗体检测经常不能直接诊断。UPT-LF试验鉴定出36份caa阳性样品，而CAA-ELISA检测到15份。综上所述，UPT-LF检测是一种复杂度较低且灵敏度高于CAA-ELISA的检测方法，适合于个体活动性血吸虫感染的实验室诊断。
Schistosoma sp. circular anodic antigen (CAA) serum concentrations reflect actual worm burden in a patient and are a valuable tool for population screening and epidemiological research. However, for the diagnosis of individual imported schistosomiasis cases, the current enzyme-linked immunosorbent assay (ELISA) lacks sensitivity and robustness. Therefore, a lateral flow (LF) assay was developed to test CAA in serum for individual diagnosis of imported active schistosome infections. Application of fluorescent submicron-sized up-converting phosphor technology (UPT) reporter particles increased analytical sensitivity compared to that of the standard ELISA method. Evaluation of the UPT-LF test with a selection of 40 characterized epidemiologic samples indicated a good correlation between signal intensity and infection intensity. Subsequently, the UPT-LF assay was applied to 166 serum samples of Dutch residents (immigrants and travelers) suspected of schistosomiasis, a case in which group routine antibody detection frequently fails straightforward diagnosis. The UPT-LF assay identified 36 CAA-positive samples, compared to 15 detected by CAA-ELISA. In conclusion, the UPT-LF assay is a low-complexity test with higher sensitivity than the CAA-ELISA, well suited for laboratory diagnosis of individual active Schistosoma infections.