MicroRNAs: small RNAs with big effects.
MicroRNAs: small RNAs with big effects.
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DOI：
10.1097/tp.0b013e3181e913c2
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发表时间：
2010-07-27
期刊：
影响因子：
6.2
通讯作者：
Suthanthiran M
中科院分区：
文献类型：
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作者：
Anglicheau D;Muthukumar T;Suthanthiran M
MicroRNA是进化上保守的小（~20-25个核苷酸）单链分子，其通过翻译抑制、信使RNA降解或两者来抑制蛋白质编码基因的表达。在人类基因组中已经发现了700多种microRNA。令人惊讶的是，单个miRNA可以调节细胞内数百种mRNA/蛋白质的表达。小RNA作为先天性和适应性免疫的主要调节因子正在迅速崛起，并且很可能在移植中发挥关键作用。RNA测序（“下一代测序”）的临床应用应有助于以前所未有的分辨率进行转录组分析。我们概述了微小RNA生物学及其在移植中的假设作用。
MicroRNAs are evolutionarily conserved, small (~20–25 nucleotides), single-stranded molecules that suppress the expression of protein-coding genes by translational repression, messenger RNA degradation, or both. More than 700 microRNAs have been identified in the human genome. Amazingly, a single miRNA can regulate the expression of hundreds of mRNAs/proteins within a cell. The small RNAs are fast emerging as master regulators of innate and adaptive immunity, and very likely to play a pivotal role in transplantation. The clinical application of RNA sequencing (“next – generation sequencing”) should facilitate transcriptome profiling at an unprecedented resolution. We provide an overview of microRNA biology and their hypothesized roles in transplantation.