Lipid production from sweet sorghum bagasse through yeast fermentation

Lipid production from sweet sorghum bagasse through yeast fermentation
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DOI:
10.1016/j.renene.2011.09.035
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发表时间:
2012-04-01
期刊:
影响因子:
8.7
通讯作者:
Umagiliyage, Arosha Loku
Umagiliyage, Arosha Loku
中科院分区:
工程技术1区
文献类型:
--
作者:
Liang, Yanna;Tang, Tianyu;Umagiliyage, Arosha Loku

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弯曲隐球菌在发酵甜高粱蔗渣的非条件水解物方面具有很大的潜力。用石灰对固体进行微波酶解,所得水解物的最大酵母细胞干重为10.83g/L,脂肪含量为73.26%。相同条件下不加石灰的水解液,这两个参数分别为15.50g/L和63.98%。在酵母发酵过程中,葡萄糖和木糖被同时消耗,而纤维二糖从残渣中释放出来。石灰的存在一方面使纤维素酶更容易被酶所利用,表现为总还原糖的释放量高于未加石灰的纤维素酶;另一方面,石灰的存在导致了糖类在预处理过程中降解为非糖化学物质。结果表明,微波预处理+酶解工艺可获得0.11g/g蔗渣或0.65吨/hm2蔗渣的较高得油率,而石灰辅助微波预处理-酶糖化工艺可获得0.09g/g蔗渣或0.51吨/hm2土地的较高得油率。(C)2011爱思唯尔有限公司。保留所有权利。
Cryptococcus curvatus has great potential in fermenting unconditioned hydrolysates of sweet sorghum bagasse. With hydrolysates obtained by enzymatic hydrolysis of the solid pretreated by microwave with lime, the maximal yeast cell dry weight and lipid content were 10.83 g/l and 73.26%, respectively. For hydrolysates obtained in the same way but without lime, these two parameters were 15.50 g/l and 63.98%, respectively. During yeast fermentation, glucose and xylose were consumed simultaneously while cellobiose was released from the residual bagasse. The presence of lime, on one hand, made cellulose more accessible to enzymes as evidenced by higher total reducing sugar release compared to that without during enzymatic hydrolysis step; on the other hand, it caused the degradation of sugars to non-sugar chemicals during pretreatment step. As a result, higher lipid yield of 0.11 g/g bagasse or 0.65 ton/hectare of land was achieved from the pathway of microwave pretreatment and enzymatic hydrolysis while 0.09 g/g bagasse or 0.51 ton/hectare of land was attained from the process of lime-assisted microwave pretreatment followed by the same enzymatic saccharification. (C) 2011 Elsevier Ltd. All rights reserved.