cDNA cloning of the beta-subunit of the rat gastric H,K-ATPase.
cDNA cloning of the beta-subunit of the rat gastric H,K-ATPase.
复制标题
DOI：
10.1016/s0021-9258(19)38317-6
复制
发表时间：
1990-07
期刊：
影响因子：
--
通讯作者：
Gary E. Shull
中科院分区：
文献类型：
--
作者：
Gary E. Shull
基于猪H，K- atp酶β -亚基的两个肽的氨基酸序列，利用寡核苷酸探针鉴定了编码大鼠胃H，K- atp酶β -亚基的cDNA （Hall， K, Perez， G., Anderson， D., Gutierrez， C., Munson， K., Hersey， S. J., Kaplan， J. H.和Sachs， G.（1990）生物化学29,701-706）。测定了该蛋白1.3千碱基cDNA的核苷酸序列，并推导出该蛋白的一级结构。该蛋白由294个氨基酸组成，Mr为33,625。H， k - atp酶β亚基的氨基酸序列与Na， k - atp酶的β 1亚基（同源性29%）和β 2亚基（同源性37%）相似。根据亲水特征，它似乎与Na， k - atp酶β亚基具有相同的跨膜组织，在氨基端附近具有单个跨膜结构域。七个潜在的n链糖基化位点位于蛋白质的假定细胞外区域。对13个组织的poly(A)+ rna的Northern blot分析表明，H， k - atp酶β亚基mRNA在胃中高水平表达，而在其他组织中不表达。
A cDNA encoding the beta-subunit of the rat gastric H,K-ATPase has been identified using oligonucleotide probes based on the amino acid sequences of two peptides from the pig H,K-ATPase beta-subunit (Hall, K., Perez, G., Anderson, D., Gutierrez, C., Munson, K., Hersey, S. J., Kaplan, J. H., and Sachs, G. (1990) Biochemistry 29, 701-706). The nucleotide sequence of the 1.3-kilobase cDNA has been determined and the primary structure of the protein deduced. The protein consists of 294 amino acids and has an Mr of 33,625. The amino acid sequence of the H,K-ATPase beta-subunit is similar to those of the beta 1 (29% identity) and beta 2 (37% identity) subunits of the Na,K-ATPase. Based on the hydropathy profile it seems to have the same transmembrane organization as the Na,K-ATPase beta-subunit, with a single membrane-spanning domain near the amino terminus. Seven potential N-linked glycosylation sites are located in the putative extracellular regions of the protein. Northern blot analyses of poly(A)+ RNAs from 13 tissues demonstrate that the H,K-ATPase beta-subunit mRNA is expressed at high level in stomach and is not expressed in any of the other tissues.