Expression and localization of P2 nucleotide receptor subtypes during development of the lateral ventricular choroid plexus of the rat.
Expression and localization of P2 nucleotide receptor subtypes during development of the lateral ventricular choroid plexus of the rat.
复制标题
大鼠侧心室脉络丛发育过程中 P2 核苷酸受体亚型的表达和定位。
DOI：
10.1111/j.1460-9568.2007.05562.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Saunders,NR
中科院分区：
文献类型：
--
作者：
Johansson,PA;Burnstock,G;Dziegielewska,KM;Guida,E;McIntyre,P;Saunders,NR
脉络膜丛分泌脑脊液（CSF），并通过血- CSF屏障调节脑内环境。血-脑脊液界面的通透性已经在成人和未成熟的大脑中进行了研究，然而，对脑脊液分泌的发展及其调节知之甚少。ATP通过嗜离子性P2X或代谢性P2Y受体影响其他上皮细胞的分泌。P2受体在出生后经常被发现下调，提示嘌呤能和嘧啶信号的发育作用。本研究研究了P2受体在侧脑室脉络膜丛中的表达，并与大鼠胚胎侧脑室水通道蛋白- 1的表达和快速扩张的最新研究相关联。在本研究中，除P2X7外，所有已知哺乳动物核苷酸受体亚型的mrna早在E15年就被鉴定出来。E18中检测到P2X7mRNA。在发育过程中观察到不同亚型的差异表达模式的迹象：p2y2和P2X7的表达明显增加，P2X1‐2,4的表达下降，p2x6和P2Y12‐13的表达水平没有可检测到的差异，P2X3,5和P2Y1,4,6,14的瞬时表达高峰。免疫组织化学方法在发育早期（E15或E18）的脉络膜上皮中检测到p2x4,5,7和p2y1,4受体蛋白。它们不同的发育特征表明它们在脑脊液分泌的发育中起着特定的作用，这可能与胚胎中脑室的快速扩张有特殊的关系。p2x5和p2y6也分别在P0和P9发育中的神经鞘瘤中检测到。
The choroid plexuses secrete cerebrospinal fluid (CSF) and regulate the brain's internal environment via the blood–CSF barrier. The permeability properties of the blood–CSF interface have been studied previously in adult and immature brains, however, little is known about the development of CSF secretion and its modulation. ATP influences secretion in other epithelia via ionotropic P2X or metabotropic P2Y receptors. P2 receptors have frequently been found to be down‐regulated in the postnatal period, suggesting a developmental role for purinergic and pyrimidine signalling. The present study investigated the expression of P2 receptors in lateral ventricular choroid plexus in relation to recent studies of aquaporin‐1 expression and rapid expansion of the lateral ventricles in rat embryos. In the present study mRNAs for all known mammalian nucleotide receptor subtypes, except P2X7, were identified from as early as E15. P2X7mRNA was detected from E18. Indications of differential expression patterns were observed for the different subtypes during development: an apparent increase in expression for P2Y2and P2X7, a decline in P2X1‐2,4, no detectable difference in expression levels for P2X6and P2Y12‐13and transient expression peaks for P2X3,5and P2Y1,4,6,14. P2X4,5,7and P2Y1,4receptor proteins were detected immunohistochemically in the choroidal epithelium from early in development (E15 or E18). Their differing developmental profiles suggest specific roles in the development of CSF secretion that may have particular relevance for the rapid expansion of the ventricles that occurs in the embryo. P2X5and P2Y6were also detected in the developing neuropendyma from P0 and P9, respectively.