CO-IMMOBILIZED NITROSOMONAS-EUROPAEA AND NITROBACTER-AGILIS CELLS - VALIDATION OF A DYNAMIC-MODEL FOR SIMULTANEOUS SUBSTRATE CONVERSION AND GROWTH IN KAPPA-CARRAGEENAN GEL BEADS
CO-IMMOBILIZED NITROSOMONAS-EUROPAEA AND NITROBACTER-AGILIS CELLS - VALIDATION OF A DYNAMIC-MODEL FOR SIMULTANEOUS SUBSTRATE CONVERSION AND GROWTH IN KAPPA-CARRAGEENAN GEL BEADS
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DOI：
10.1002/bit.260431121
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发表时间：
1994-05-01
影响因子：
3.8
通讯作者：
TRAMPER, J
中科院分区：
文献类型：
--
作者：
HUNIK, JH;BOS, CG;TRAMPER, J
建立了两种微生物在凝胶基质中固定化的动力学模型，并通过实验进行了验证。该模型描述了在kappa- carragenan凝胶珠中共同固定europaea亚硝基单胞菌和agile亚硝基杆菌对氨的硝化作用。该模型由微生物的动力学方程和凝胶珠内外底物及产物的传质方程组成。该模型预测反应器的总体浓度以及底物消耗率、产物形成和凝胶珠内生物量的生长作为时间的函数。在3.3 dm(3)气升式循环反应器中进行了为期50天的固定细胞实验，以验证该模型。模型的参数值来源于文献和单独的实验。实验测定的反应器体积浓度和凝胶珠中两种微生物的生物量分布都能很好地预测。对给定初始值的模型进行敏感性分析表明，最相关的参数是微生物的最大比生长率、氧气扩散系数和微球半径。该动态模型为进一步研究和控制硝化过程提供了有用的工具。(C) 1994 John Wiley & Sons, Inc。
A dynamic model for two microbial species immobilized in a gel matrix is presented and validated with experiments. The model characterizes the nitrification of ammonia with Nitrosomonas europaea and Nitrobacter agilis co-immobilized in kappa-carrageenan gel beads. The model consists of kinetic equations for the microorganisms and mass transfer equations for the substrates and products inside and outside the gel beads. The model predicts reactor bulk concentrations together with the substrate consumption rate, product formation, and biomass growth inside the gel beads as a function of time. A 50-day experiment with immobilized cells in a 3.3-dm(3) air-lift loop reactor was carried out to validate the model. The parameter values for the model were obtained from literature and separate experiments. The experimentally determined reactor bulk concentrations and the biomass distribution of the two microorganisms in the gel beads were well predicted by the model. A sensitivity analysis of the model for the given initial values indicated the most relevant parameters to be the maximum specific growth rate of the microorganisms, the diffusion coefficient of oxygen, and the radius of the beads. The dynamic model provides a useful tool for further study and possible control of the nitrification process. (C) 1994 John Wiley & Sons, Inc.