A converging subset of soil bacterial taxa is permissive to the IncP-1 plasmid pKJK5 across a range of soil copper contamination
A converging subset of soil bacterial taxa is permissive to the IncP-1 plasmid pKJK5 across a range of soil copper contamination
复制标题
土壤细菌类群的聚合子集允许 IncP-1 质粒 pKJK5 跨越一系列土壤铜污染
DOI：
10.1093/femsec/fiaa200
复制
发表时间：
2020
影响因子：
4.2
通讯作者：
t
中科院分区：
文献类型：
--
作者：
Jianxiao Song;Uli Klümper;Leise Riber;Arnaud Dechesne;Barth F Smets;Søren J Sørensen;Kristian K Br;t
抽象的。金属或抗生素等应激源可以影响细菌群落对质粒摄取的迁移性，但关于这种应激源对群落迁移性演变的长期影响知之甚少。我们评估了90多年的土壤铜污染对细菌群落对通过大肠杆菌供体导入的GFP标记的IncP-1质粒(PKJK5)的透过性(即摄取能力)的影响。对从供体到受体土壤细菌群落的质粒转移事件进行量化，然后通过荧光激活细胞分选分离转接子，并通过16S rRNA基因扩增片段测序进行鉴定。从高铜污染(4526 mg kg−1)土壤中提取的细菌群落中的pKJK5转移频率低于从中铜污染(458 mg kg−1)土壤和低铜参考土壤(15 mg kg−1)中提取的相应群落。无论土壤铜污染的程度如何，尽管提取的受体群落和原始土壤群落的组成不同，但转合群落的分类组成显示出显着的相似性。在单个操作分类单位(OTUS；16S rRNA基因97%序列相似性阈值)水平上评估的透过性仅受土壤铜水平的轻微影响，OTU水平透过性的高重复变异性表明随机事件在IncP-1质粒转移或菌株之间的透过性变异性中起作用。
ABSTRACT. Stressors like metals or antibiotics can affect bacterial community permissiveness for plasmid uptake, but there is little knowledge about long-term effects of such stressors on the evolution of community permissiveness. We assessed the effect of more than 90 years of soil Cu contamination on bacterial community permissiveness (i.e. uptake ability) toward a gfp-tagged IncP-1 plasmid (pKJK5) introduced via an Escherichia coli donor. Plasmid transfer events from the donor to the recipient soil bacterial community were quantified and transconjugants were subsequently isolated by fluorescence activated cell sorting and identified by 16S rRNA gene amplicon sequencing. Transfer frequency of plasmid pKJK5 was reduced in bacterial communities extracted from highly Cu contaminated (4526mg kg−1) soil compared to corresponding communities extracted from moderately (458mg kg−1) Cu contaminated soil and a low Cu reference soil (15mg kg−1). The taxonomic composition of the transconjugal pools showed remarkable similarities irrespective of the degree of soil Cu contamination and despite contrasting compositions of the extracted recipient communities and the original soil communities. Permissiveness assessed at the level of individual operational taxonomic units (OTUs; 16S rRNA gene 97% sequence similarity threshold) was only slightly affected by soil Cu level and high replicate variability of OTU-level permissiveness indicated a role of stochastic events in IncP-1 plasmid transfer or strain-to-strain permissiveness variability.