SOME PROPERTIES OF PYOVERDINE, THE WATER-SOLUBLE FLUORESCENT PIGMENT OF THE PSEUDOMONADS
SOME PROPERTIES OF PYOVERDINE, THE WATER-SOLUBLE FLUORESCENT PIGMENT OF THE PSEUDOMONADS
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DOI：
10.1128/aem.6.4.241-246.1958
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发表时间：
1958-01-01
期刊：
影响因子：
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通讯作者：
ELLIOTT, RP
中科院分区：
文献类型：
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作者：
ELLIOTT, RP
术语“pyoverdine”（P）被认为是该颜料的第一个定义术语，而术语“荧光素”和“荧光素”（P 通常被称为）分别与不相关的化学物质间苯二酚酞和间苯二酚酞同义。P 的粗制制剂不会结晶，也没有精确的熔点。它在 404-412 mu 处有一个强吸收峰，荧光发射峰随 pH 值变化约450 mu 至 550 mu。它不含游离核黄素。当氧化还原电位降至-0.3 伏时，P 的形成就会停止，但可见光不会分解；但即使在黑暗中储存数周，它也不会分解。金黄色葡萄球菌或大肠杆菌的菌株始终产生蓝色荧光壳卵，并且磷含量低，而卵形假单胞菌菌株则始终产生黄绿色荧光壳卵，并且磷含量高。低浓度的磷在长波紫外线下呈蓝色，而高浓度的磷则呈黄绿色。
The term pyoverdine" (P) is recalled as the 1st definitive term for this pigment, whereas the terms "fluorescin" and "fluorescein", as P is often called, are synonymous with the unrelated chemicals resorcinolphthalin and resorcinolphthalein, respectively. A crude preparation of P would not crystallize and had no precise melting point. It has a strong absorption peak at 404-412 mu, and fluorescence emission peaks varying with pH from about 450 mu to 550 mu. P is a reversible pH indicator. It contains no free riboflavin. Fluorescence and absorption are proportional to concentration. P formation requires oxygen. Formation ceased in spoiling whole egg meat medium when the oxidation-reduction potential dropped to -0.3 volts. Ultraviolet light causes P to break down, but visible light does not; however, it will break down even in the dark if stored several weeks. It has no antibiotic activity against Micrococcus pyogenes var. aureus or Escherichia coli. A strain of Pseudomonas fluorescens produced blue fluorescent shell eggs consistently, and low quantities of P, whereas a strain of Pseudomonas ovalis produced yellow-green fluorescent shell eggs consistently and high quantities of P. Low concentrations of P are blue under long wave UV light, whereas high concentrations are yellow-green.