Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings
Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings
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DOI：
10.1086/342663
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发表时间：
2002-09-01
影响因子：
6.4
通讯作者：
Dardé, ML
中科院分区：
文献类型：
--
作者：
Ajzenberg, D;Cogné, N;Dardé, ML
为了研究弓形虫基因型对人类先天性弓形虫病严重程度（无症状、良性、重度感染或新生儿或胎儿死亡）的影响，采用8个微卫星标记对86株先天性弓形虫患者的分离株进行了分析。检测到74种不同的基因型，一些相同的基因型可能来自同一污染源。与6个同工酶标记相关的3个多态性较少的微卫星标记将分离物分为3种经典型和检测到的非典型基因型。无论临床表现如何，II型菌株均占绝大多数（全部标本占84.88%，连续57例病例占96.49%）。在无症状或良性先天性弓形虫病中未发现I型和非典型分离株。然而，在从胎盘中分离出弓形虫的4例儿童未感染的病例中，只发现了I型分离株。
To study the influence of Toxoplasma gondii genotypes on the severity of human congenital toxoplasmosis (asymptomatic, benign, or severe infection or newborn or fetal death), 8 microsatellite markers were used to analyze 86 T. gondii isolates collected from patients with congenital toxoplasmosis. Seventy-four different genotypes were detected, some identical genotypes originating probably from the same source of contamination. The 3 less polymorphic microsatellite markers associated with 6 isoenzymatic markers allowed a classification of isolates into the 3 classical types and detected atypical genotypes. Whatever the clinical findings, type II isolates were largely predominant (84.88% in the whole collection and 96.49% in 57 consecutive cases). Type I and atypical isolates were not found in asymptomatic or benign congenital toxoplasmosis. However, in 4 cases in which children were not infected despite isolation of T. gondii from placenta, only type I isolates were found.