Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene.
Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene.
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xisA 基因失活可阻断鱼腥藻的基因组重排和固氮作用。
DOI：
10.1126/science.3144039
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Wiest,DR
中科院分区：
文献类型：
--
作者：
Golden,JW;Wiest,DR
在鱼腥藻光合营养细胞向固氮异形胞的分化过程中，发生了两种涉及11-和55-组氨酸酶DNA元件的基因组重排。位于thenifD 11-脱氢酶DNA元件上的thenifA基因通过染色体与插入抗生素基因盒突变的thenifA基因拷贝之间的重组而失活。定点失活theAnabaenaxisA基因阻断重排的11-β-淀粉酶元件和固氮，但不影响重排的55-β-淀粉酶元件，异形胞分化，或异形胞模式的形成。
Two genome rearrangements involving 11- and 55-kilobase DNA elements occur during the terminal differentiation of anAnabaenaphotosynthetic vegetative cell into a nitrogen-fixing heterocyst. ThexisAgene, located on thenifD11-kilobase DNA element, was inactivated by recombination between the chromosome and a copy of thexisAgene that was mutated by inserting an antibiotic gene cassette. Site-directed inactivation of theAnabaenaxisAgene blocked rearrangement of the 11-kilobase element and nitrogen fixation, but did not affect rearrangement of the 55-kilobase element, heterocyst differentiation, or heterocyst pattern formation.