Pancreatic Autoantibodies Against CUZD1 and GP2 Are Associated with Distinct Clinical Phenotypes of Crohn's Disease
Pancreatic Autoantibodies Against CUZD1 and GP2 Are Associated with Distinct Clinical Phenotypes of Crohn's Disease
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DOI：
10.1097/mib.0000000000000564
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发表时间：
2015-12-01
影响因子：
4.9
通讯作者：
Sina, Christian
中科院分区：
文献类型：
--
作者：
Michaels, Maike Anna;Jendrek, Sebastian Torben;Sina, Christian
背景：炎症性肠病（IBD）的特点是具有广泛的临床表型和不同的结果。在过去的几十年中，已经鉴定并研究了几种 IBD 相关自身抗体的诊断相关性。针对胰腺糖蛋白 (PAB) CUB 和透明带样结构域的蛋白 1 (CUZD1) 和糖蛋白 2 (GP2) 的自身抗体已被证明对诊断 IBD 具有高度特异性。尽管一些研究表明抗 GP2 阳性与疾病表型存在显着的相互关系，但临床表型与抗 CUZD1 的关联仍不清楚。目的是在明确的德国 IBD 队列中确定临床表型与抗 CUZD1 和抗 GP2 的关联。方法：2005 年至 2005 年间，通过对转染细胞进行间接免疫荧光法，对 IBD 患者（224 名克罗恩病患者和 136 名溃疡性结肠炎患者）进行抗 GP2 和抗 CUZD1 免疫球蛋白 G 和免疫球蛋白 A 检测。 2013年，被纳入其中。回顾性收集血清型和特定表型数据并进行统计分析。结果：抗GP2（P < 0.001）和抗CUZD1（P < 0.001）在克罗恩病患者中的患病率显着高于溃疡性结肠炎患者。 PAB 阳性与回结肠疾病 (P = 0.002)、肛周疾病 (P = 0.011)、免疫抑制治疗 (P = 0.036) 和 ASCA 阳性 (P = 0.036) 相关。抗 CUZD1 阳性与回结肠 (P = 0.016) 和肛周疾病 (P = 0.002) 相关，而抗 GP2 阳性与狭窄行为呈正相关 (P = 0.016)。 结论：我们发现不同的临床表型与 PAB 阳性相关。因此，PAB 的测定及其亚组分析可能会识别患有复杂疾病行为的患者。然而，我们的研究结果的临床相关性应该在前瞻性队列中进一步评估。
Background:Inflammatory bowel disease (IBD) is characterized by a broad spectrum of clinical phenotypes with different outcomes. In the last decades, several IBD-associated autoantibodies have been identified and investigated for their diagnostic relevance. Autoantibodies against the pancreatic glycoproteins (PAB) CUB and zona pellucida-like domains-containing protein 1 (CUZD1), and glycoprotein 2 (GP2) have been demonstrated to possess high specificity for the diagnosis of IBD. Although several studies have shown significant interrelations of anti-GP2 positivity with disease phenotype, associations of clinical phenotypes with anti-CUZD1 are still unknown. The aim was to identify the association of clinical phenotypes with anti-CUZD1 and anti-GP2 in a well-defined German IBD cohort.Methods:Patients with IBD (224 patients with Crohn's disease and 136 patients with ulcerative colitis), who were tested for anti-GP2 and anti-CUZD1 immunoglobulin G and immunoglobulin A by indirect immunofluorescence on transfected cells between 2005 and 2013, were included. Serotype and specified phenotypic data were collected in retrospect and statistically analyzed.Results:Both anti-GP2 (P < 0.001) and anti-CUZD1 (P < 0.001) were significantly more prevalent in patients with Crohn's disease than in ulcerative colitis. PAB positivity was associated with ileocolonic disease (P = 0.002), perianal disease (P = 0.011), immunosuppressive treatment (P = 0.036), and ASCA positivity (P = 0.036). Anti-CUZD1 positivity was associated with ileocolonic (P = 0.016) and perianal disease (P = 0.002), whereas anti-GP2 positivity was positively associated with stricturing behavior (P = 0.016).Conclusions:We found distinct clinical phenotypes to be associated with PAB positivity. Therefore, determination of PABs and their subgroup analysis might identify patients with complicated disease behavior. However, the clinical relevance of our findings should be further evaluated in prospective cohorts.